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. 2017 Mar 15;36(6):783-796.
doi: 10.15252/embj.201593378. Epub 2017 Feb 20.

Hsp70 displaces small heat shock proteins from aggregates to initiate protein refolding

Szymon Żwirowski  1 , Agnieszka Kłosowska  1 , Igor Obuchowski  1 , Nadinath B Nillegoda  2   3 , Artur Piróg  1 , Szymon Ziętkiewicz  1 , Bernd Bukau  2   3 , Axel Mogk  2   3 , Krzysztof Liberek  4
Affiliations

Hsp70 displaces small heat shock proteins from aggregates to initiate protein refolding

Szymon Żwirowski et al. EMBO J. .

Abstract

Small heat shock proteins (sHsps) are an evolutionary conserved class of ATP-independent chaperones that protect cells against proteotoxic stress. sHsps form assemblies with aggregation-prone misfolded proteins, which facilitates subsequent substrate solubilization and refolding by ATP-dependent Hsp70 and Hsp100 chaperones. Substrate solubilization requires disruption of sHsp association with trapped misfolded proteins. Here, we unravel a specific interplay between Hsp70 and sHsps at the initial step of the solubilization process. We show that Hsp70 displaces surface-bound sHsps from sHsp-substrate assemblies. This Hsp70 activity is unique among chaperones and highly sensitive to alterations in Hsp70 concentrations. The Hsp70 activity is reflected in the organization of sHsp-substrate assemblies, including an outer dynamic sHsp shell that is removed by Hsp70 and a stable core comprised mainly of aggregated substrates. Binding of Hsp70 to the sHsp/substrate core protects the core from aggregation and directs sequestered substrates towards refolding pathway. The sHsp/Hsp70 interplay has major impact on protein homeostasis as it sensitizes substrate release towards cellular Hsp70 availability ensuring efficient refolding of damaged proteins under favourable folding conditions.

Keywords: Hsp100 disaggregase; Hsp70; protein aggregation; protein refolding; sHsps.

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Figures

Figure 1
Figure 1. IbpAB–luciferase assemblies differ in biophysical and biochemical properties from luciferase aggregates
  1. A

    Dynamic light scattering graphs depicting the size distribution of the following entities: native luciferase (green), IbpAB (grey), luciferase aggregates (red), IbpAB–luciferase assemblies (blue), shown as volume distribution of analysed species. Luciferase was present at 1.5 μM, IbpA at 3 μM, IbpB at 7 μM concentration.

  2. B

    Scheme of the disaggregation experiment.

  3. C, D

    Renaturation kinetics for luciferase denatured in the presence or absence of IbpAB. Luciferase (1.5 μM) was denatured in the presence of IbpA (3 μM) and IbpB (7 μM), diluted to 40 nM and refolded at limited (0.3 μM; C) or elevated (2.4 μM; D) KJE machinery concentrations and standard (1.5 μM) ClpB concentration. The KJE machinery concentration is given with respect to DnaK with a constant DnaK:DnaJ:GrpE ratio 1:0.3:0.3. Data are the mean ± SD of three independent experiments.

  4. E

    A comparison of renaturation rates for luciferase denatured in the presence or absence of IbpAB in changing KJE and constant ClpB concentrations. The KJE machinery concentration is given with respect to DnaK with a constant DnaK:DnaJ:GrpE ratio 1:0.3:0.3.

  5. F

    A comparison of renaturation rates for luciferase denatured in the presence or absence of IbpAB in changing ClpB and constant KJE concentrations. The dashed line indicates the theoretical scenario that luciferase refolding from aggregates and sHsp assemblies proceeds with identical refolding rates.

Figure EV1
Figure EV1. Sedimentational analysis of IbpAB–luciferase assemblies

  1. Comparison of sedimentational properties of IbpAB–luciferase assemblies and luciferase aggregates. Luciferase (1.5 μM)(upper gel), IbpA (3 μM) and IbpB (7 μM)(middle gel) or luciferase (1.5 μM), IbpA (3 μM) and IbpB (7 μM)(lower gel) were incubated at 48°C for 10 min and subjected to glycerol gradient sedimentation (SW 60 Ti rotor, 40,000 rpm, 1 h, 10°C). Fractions were collected from the top of the gradient. Proteins in the fractions were visualized by SYPRO Ruby staining following SDS–PAGE.

  2. Stoichiometry of the proteins in the IbpAB–luciferase assemblies. Fractions marked with an asterisk containing IbpAB–luciferase assemblies were pooled and following SDS–PAGE and SYPRO Ruby staining, a densitometric analysis was performed. The relative molar ratio of proteins in assemblies was calculated by taking into account the respective molecular masses.

Source data are available online for this figure.
Figure 2
Figure 2. KJE acts prior to the disaggregation step of IbpAB–luciferase assemblies
An order of addition experiment. Luciferase (1.5 μM) was denatured in the presence of IbpA (3 μM) and IbpB (7 μM), diluted to 40 nM and incubated first for 20 min with the components listed in the legend and then complemented with the missing components of the functional ClpB‐KJE bichaperone system. The DnaK, DnaJ, GrpE, ClpB concentrations used were as follows: 1, 0.3, 0.3 and 1.5 μM. Data are the mean ± SD of three independent experiments.
Figure EV2
Figure EV2. KJE acts prior to disaggregation of IbpAB–MDH assemblies
An order of addition experiment. MDH (2 μM) was denatured in the presence of IbpA (3 μM) and IbpB (7 μM) at 47°C for 30 min. IbpAB–MDH assemblies were diluted fourfold and first incubated with the components listed in the legend and then complemented with the missing components of the functional ClpB‐KJE bichaperone system. The DnaK, DnaJ, GrpE, ClpB concentrations used were as follows: 1, 0.3, 0.3 and 1.5 μM. Data are the mean ± SD of three independent experiments.
Figure 3
Figure 3. Hsp70, independently of Hsp100, releases IbpAB‐dependent inhibition of disaggregation

  1. IbpAB association with GFP aggregates blocks substrate translocation through the central Hsp100 pore. Aggregated GFP (1.5 μM) was incubated for 10 min at 48°C (marked “T” in the Figure) alone (blue) or with IbpA and IbpB (3 μM and 7 μM, respectively; sky blue) or aggregated GFP and IbpAB were incubated separately at 48°C and then mixed (orange). Hsp104 D484K hyperactive variant (1 μM) was added, and GFP reactivation was monitored.

  2. KJE machinery allows to overcome the IbpAB‐dependent inhibition of IbpAB–GFP disaggregation by hyperactive Hsp104 D484K. Aggregated GFP was incubated alone at 48°C (blue and orange) or with IbpAB at 48°C (sky blue and black). After addition of the Hsp104 D484K hyperactive mutant (all plots) and KJE machinery (black and magenta), GFP reactivation was monitored. DnaK, DnaJ and GrpE were present at 1 μM, 0.3 μM and 0.3 μM. Aggregated GFP, IbpAB and Hsp104 D484K were in concentrations as in (A).

Figure 4
Figure 4. Hsp70 releases sHsps from sHsp–substrate assemblies in vitro

  1. Experimental scheme. Purified IbpAB–luciferase assemblies were incubated with indicated components for the indicated time and resubjected to glycerol gradient sedimentation.

  2. KJE releases IbpAB from IbpAB–luciferase assemblies. Fractions were collected from the top of the gradient, and luciferase and IbpA were visualized by Western blot following SDS–PAGE. The band intensities of luciferase and IbpA retained in the core assembly (fractions 5–12) or released from the assembly (fractions 1–3) were quantified and plotted on the graphs on the left. IbpA–IbpB–luciferase assemblies (approximate stoichiometry 1:0.8:1), DnaK, DnaJ and GrpE were present at 100 nM (calculated for luciferase monomer), 1 μM, 0.3 μM and 0.3 μM concentrations, respectively.

  3. Concerted action of ClpB‐KJE results in release of IbpAB and luciferase from IbpAB–luciferase assemblies. Experiments were performed and analysed as in (B). ClpB was present at 1.5 μM concentration.

Source data are available online for this figure.
Figure EV3
Figure EV3. Hsp70 specifically displaces sHsps sHsp–substrate assemblies

  1. KJE requires ATP to release IbpAB from IbpAB–luciferase assemblies. The isolated assemblies were incubated with the indicated components for 60 min and resubjected to glycerol gradient sedimentation as in Fig 4B, but instead of showing the entire gradients, the fractions corresponding to the released material (1–3) were pooled together and IbpA was visualized by Western blot following SDS–PAGE.

  2. An order of addition experiment as in Fig 2. IbpAB–luciferase assemblies were first incubated with the components listed in the legend and then complemented with the missing components of the functional ClpB‐KJE bichaperone system. The DnaK, DnaJ, GrpE and ClpB concentrations used were as follows: 1, 0.6, 0.3 and 1.5 μM. Data are the mean ± SD of three independent experiments.

  3. Incubation of the IbpAB–luciferase assemblies in the presence of DnaJ alone does not lead to IbpAB release. Experiment performed as in (A).

  4. Incubation of the IbpAB–luciferase assemblies in the presence of Hsp100 family members does not lead to IbpAB release. Experiments were performed as in (A). ClpB, ClpC, MecA, ClpA and BSA were present at 1 μM concentration.

Source data are available online for this figure.
Figure EV4
Figure EV4. KJE dissociates IbpAB from IbpAB–MDH assemblies in vitro

  1. Experimental scheme. IbpAB–MDH assemblies, isolated from unbound IbpAB via sedimentation in glycerol gradient, were incubated with the indicated components for 120 min and resubjected to a second round of sedimentation.

  2. KJE dissociates IbpAB from IbpAB–MDH assemblies. Fractions were collected from the top of the gradient, and MDH and IbpA were visualized by Western blot following SDS–PAGE. IbpA and MDH levels in each fraction were determined by quantitative immunoblot analysis using IbpA‐ and MDH‐specific antibodies, respectively. The band intensities of IbpA and MDH retained in the core assembly (fractions 5–12) or released from the assembly (fractions 1–3) were quantified and plotted on the graphs on the left. IbpAB–MDH assemblies (approximate stoichiometry 1:0.5:1), DnaK, DnaJ, GrpE and ClpB were present at 400 nM (calculated for MDH monomers), 1 μM, 0.3 μM, 0.3 μM and 1.5 μM concentrations, respectively.

Source data are available online for this figure.
Figure 5
Figure 5. sHsps spontaneously dissociate from and re‐associate with sHsp–substrate assemblies
Surface‐exposed IbpAB are dynamically binding and dissociating from the assemblies. Purified IbpAB–luciferase assemblies (100 nM; calculated for luciferase monomer; approximate stoichiometry 1:0.8:1) were incubated with the indicated peptides (1 mM each) for 60 min and subjected to glycerol gradient sedimentation. Fractions were collected from the top of the gradient, and luciferase and IbpA were visualized by Western blot following SDS–PAGE. Source data are available online for this figure.
Figure 6
Figure 6. Hsp70 remains associated with the assembly following sHsp displacement

  1. Purified IbpAB–luciferase assemblies were incubated with buffer or KJE and 2 mM ATP at 25°C for 60 min and resubjected to glycerol gradient sedimentation. Fractions were collected from the top of the gradient, and luciferase and DnaK were visualized by Western blot analysis. To avoid visualizing dominant, unbound DnaK, only the lower, IbpAB–luciferase assembly‐containing fractions of the gradient are shown. Sedimentation analysis of KJE only is provided as a control. IbpAB–luciferase assemblies (approximate stoichiometry 1:0.8:1), DnaK, DnaJ and GrpE were present at 100 nM (calculated for luciferase monomer), 1 μM, 0.3 μM and 0.3 μM concentrations, respectively.

  2. DnaK binding to IbpAB–luciferase assemblies is sufficient to release IbpAB. Purified IbpAB–luciferase assemblies (100 nM) were incubated with the indicated components for 60 min and subjected to glycerol gradient sedimentation. Fractions were collected from the top of the gradient and IbpA was visualized by Western blot following SDS–PAGE.

  3. Purified IbpAB–luciferase assemblies (100 nM) were incubated for 60 min with buffer or DnaK (10 μM) or DnaK V436F (10 μM) and subjected to glycerol gradient sedimentation as in Fig 4B, but instead of showing the entire gradients, the fractions corresponding to the released material (1–3) were pooled together and IbpA was visualized by Western blot following SDS–PAGE.

Source data are available online for this figure.
Figure 7
Figure 7. Hsp70 releases sHsps from the fraction of aggregated proteins in vivo

  1. Experimental scheme. BB6410 (PA1lacO‐1) dnaK, dnaJ, lacIq, ΔclpB cells were grown at 30°C in LB medium until late log phase, heat‐shocked at 46°C for 30 min, then the culture was separated into two flasks and IPTG was added at 1 mM. At indicated time points, aliquots were taken, cells were lysed and protein aggregates were isolated.

  2. The isolated fraction of aggregates was analysed by SDS–PAGE followed by staining with Coomassie brilliant blue at the indicated time points.

  3. Levels of IbpA present in aggregates at the indicated time points were determined by quantitative immunoblot analysis using IbpA‐specific antibodies.

  4. Quantification of (B) and (C).

  5. DnaK cellular levels were determined by quantitative immunoblot analysis using DnaK‐specific antibodies.

Source data are available online for this figure.
Figure 8
Figure 8. Eukaryotic Hsp70 dissociates eukaryotic sHsps from sHsp–substrate assemblies in vitro

  1. Yeast Hsp70 system acts prior to disaggregation of Hsp26–MDH assemblies. An order of addition experiment. MDH (5 μM) denatured in the presence of Hsp26 (5 μM) was diluted 10‐fold and first incubated with the components listed in the legend and then complemented with the missing components of the functional Hsp104‐SYS (Ssa1, Ydj1, Sse1) bichaperone system present at 1.5 μM, 4 μM, 2 μM and 0.15 μM concentrations, respectively. Data are the mean ± SD of three independent experiments.

  2. Experimental scheme. Purified Hsp26–MDH assemblies were incubated with indicated components and subjected to glycerol gradient sedimentation.

  3. SYS (Ssa1, Ydj1, Sse1) dissociates Hsp26 from Hsp26–MDH assemblies. Fractions were collected from the top of the gradient, and MDH and Hsp26 were visualized by Western blot following SDS–PAGE. The band intensities of MDH and Hsp26 retained in the core assembly (fractions 5–12) or released from the assembly (fractions 1–3) were quantified and presented on the graphs on the left. Hsp26–MDH assemblies (approximate stoichiometry 1.25:1), Ssa1, Ydj1, Sse1 and Hsp104 were present at 400 nM (calculated for MDH monomers), 4 μM, 2 μM, 0.15 μM and 1.5 μM concentrations, respectively.

Source data are available online for this figure.
Figure EV5
Figure EV5. Prokaryotic Hsp70 dissociates eukaryotic sHsps from sHsp–substrate assemblies in vitro

  1. Experimental scheme. Hsp26–MDH assemblies isolated from unbound Hsp26 via sedimentation in a glycerol gradient were incubated with the indicated components for 120 min and resubjected to a second round of sedimentation.

  2. DnaK, DnaJ, GrpE dissociate Hsp26 from Hsp26–MDH assemblies. Fractions were collected from the top of the gradient and MDH and Hsp26 were visualized by Western blot following SDS–PAGE. MDH and Hsp26 levels in each fraction were determined by quantitative immunoblot analysis using MDH‐ and Hsp26‐specific antibodies, respectively. The band intensities of Hsp26 and MDH retained in the core assembly (fractions 5–12) or released from the assembly (fractions 1–3) were quantified and presented on the graphs on the left. Hsp26–MDH assemblies (approximate stoichiometry 1.25:1), DnaK, DnaJ, GrpE and ClpB were present at 400 nM (calculated for MDH monomers), 1 μM, 0.3 μM, 0.3 μM and 1.5 μM concentrations, respectively.

Source data are available online for this figure.
Figure 9
Figure 9. Schematic model of the Hsp100‐Hsp70 bichaperone system action on sHsp–substrate assemblies
sHsp–substrate assemblies consist of a stable sHsp–substrate core and an dynamic outer shell, comprising the majority of sHsps. Surface‐exposed sHsps are dynamically binding and dissociating from the sHsp–substrate assemblies, with the equilibrium shifted towards the bound state. Hsp70 outcompetes sHsps in binding to the assembly surface, protecting the sHsp–substrate core and rendering sHsps in the unbound state. Once the sHsps are displaced from the surface, the misfolded polypeptides are extracted from the assemblies and refolded by a concerted action of the Hsp100‐Hsp70 bichaperone system.
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