m6A-dependent maternal mRNA clearance facilitates zebrafish maternal-to-zygotic transition

doi:10.1038/nature21355

. 2017 Feb 23;542(7642):475-478.

doi: 10.1038/nature21355. Epub 2017 Feb 13.

m⁶A-dependent maternal mRNA clearance facilitates zebrafish maternal-to-zygotic transition

Boxuan Simen Zhao^{1

2}, Xiao Wang^{1

2}, Alana V Beadell³, Zhike Lu^{1

2}, Hailing Shi^{1

2}, Adam Kuuspalu³, Robert K Ho³, Chuan He^{1

2

4}

Affiliations

¹ Department of Chemistry and Institute for Biophysical Dynamics, The University of Chicago, 929 East 57th Street, Chicago, Illinois 60637, USA.
² Howard Hughes Medical Institute, The University of Chicago, 929 East 57th Street, Chicago, Illinois 60637, USA.
³ Department of Organismal Biology and Anatomy, The University of Chicago, 1027 East 57th Street, Chicago, Illinois 60637, USA.
⁴ Department of Biochemistry and Molecular Biology, The University of Chicago, 929 East 57th Street, Chicago, Illinois 60637, USA.

PMID: 28192787
PMCID: PMC5323276
DOI: 10.1038/nature21355

m⁶A-dependent maternal mRNA clearance facilitates zebrafish maternal-to-zygotic transition

Boxuan Simen Zhao et al. Nature. 2017.

. 2017 Feb 23;542(7642):475-478.

doi: 10.1038/nature21355. Epub 2017 Feb 13.

Authors

Boxuan Simen Zhao^{1

2}, Xiao Wang^{1

2}, Alana V Beadell³, Zhike Lu^{1

2}, Hailing Shi^{1

2}, Adam Kuuspalu³, Robert K Ho³, Chuan He^{1

2

4}

Affiliations

¹ Department of Chemistry and Institute for Biophysical Dynamics, The University of Chicago, 929 East 57th Street, Chicago, Illinois 60637, USA.
² Howard Hughes Medical Institute, The University of Chicago, 929 East 57th Street, Chicago, Illinois 60637, USA.
³ Department of Organismal Biology and Anatomy, The University of Chicago, 1027 East 57th Street, Chicago, Illinois 60637, USA.
⁴ Department of Biochemistry and Molecular Biology, The University of Chicago, 929 East 57th Street, Chicago, Illinois 60637, USA.

PMID: 28192787
PMCID: PMC5323276
DOI: 10.1038/nature21355

Abstract

The maternal-to-zygotic transition (MZT) is one of the most profound and tightly orchestrated processes during the early life of embryos, yet factors that shape the temporal pattern of vertebrate MZT are largely unknown. Here we show that over one-third of zebrafish maternal messenger RNAs (mRNAs) can be N⁶-methyladenosine (m⁶A) modified, and the clearance of these maternal mRNAs is facilitated by an m⁶A-binding protein, Ythdf2. Removal of Ythdf2 in zebrafish embryos decelerates the decay of m⁶A-modified maternal mRNAs and impedes zygotic genome activation. These embryos fail to initiate timely MZT, undergo cell-cycle pause, and remain developmentally delayed throughout larval life. Our study reveals m⁶A-dependent RNA decay as a previously unidentified maternally driven mechanism that regulates maternal mRNA clearance during zebrafish MZT, highlighting the critical role of m⁶A mRNA methylation in transcriptome switching and animal development.

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Conflict of interest statement

The authors declare no competing financial interests.

Figures

**Extended Data Figure 1. Generation and characterization of *ythdf2* mutant fish**
a, Design of transcription activator-like effectors (TALEs) used to generate *ythdf2* mutant fish. An 8 bp deletion upstream of the YTH RNA-binding domain is made to create a premature stop codon. b, Photos of adult wild-type and *ythdf2*^−/− mutant fish. Homozygous *ythdf2* mutant males are often smaller than wild-type males. c, Table showing the percent of embryos suffering from MBT-period developmental delay and lethality for crosses between different homozygous genotypes. Homozygous mutant females produce ~95% embryos that suffer from MBT-period developmental delay, regardless of paternal genotype. Homozygous mutant males produce on average ~70% embryos that do not develop past the 1-cell stage, regardless of maternal genotype. These two phenotypes can segregate independently from one another and reveal that Ythdf2 is pleiotropic in zebrafish development (see Supplementary Discussion). Each row is tabulated from six to eighteen crosses, scoring between 365 and 1026 embryos, and using randomly selected males and females from each genotype from at least 4 fish of each sex. d, Table showing additional crosses to characterize the paternal-associated lethality and/or sterility of *ythdf2* loss of function. Heterozygous males produce about half the number 1-cell arrested offspring as homozygous mutant males, perhaps indicating a function for Ythdf2 in later gametogenesis; this arrest occurs without respect to maternal genotype. Data for each row is tabulated from 5 crosses each, using randomly selected males and females from each genotype from at least 4 fish of each sex.

**Extended Data Figure 2. Phenotypes of maternal *ythdf2^−/−* mutant embryos**
a, Still images taken from the time-lapse movie of a maternal *ythdf2^−/−* mutant embryo filmed between 2.25 h.p.f. and 6.55 h.p.f. highlighting the development delay phenotype occurring during the MBT period. Red texts indicate stages experiencing the delay. All cell divisions are normal in mutant embryos until the cell cycle that should take place between high and oblong stages, cell cycle 12. The high stage in mutant embryos lasts ~ 60 min while the similar stage in wild-type lasts ~ 20 min. Maternal *ythdf2^−/−* mutants lose another ~ 30 min of developmental time between the pseudo-dome and pseudo-50% epiboly stages before reaching the 50% epiboly stage at 6.5 h.p.f. versus 5 h.p.f. in wild-type. b, Injection of *ythdf2* mRNA allows partial rescue of the *ythdf2* mutant phenotype. Maternal *ythdf2^−/−* mutant embryos injected with 150pg *ythdf2* mRNA showed an overall reduction in the cell cycle 12 delay compared to uninjected embryos of the same genotype. In these partially rescued embryos, an appreciable number of cells during cell cycle 12 divided at the same time as wild-type cells did. This partial rescue is nearly 100% penetrant (n=69, four experiments). c, Immunofluorescent microscopy of wild-type, *ythdf2* mutant, and *ythdf2* mRNA-injected mutant embryos fixed at 3 hours 50 min post-fertilization and stained with anti-phospho-histone H3 (pH3) antibody to label nuclei in late G2 phase through M-phase of the cell cycle. The bar chart shows the average number of condensed pH3-positive nuclei per 100 cells. The results showed that unlike wild-type embryos, maternal *ythdf2^−/−* mutants are lacking in the tight, condensed pH3 nuclei staining characteristic of metaphase/anaphase, which can be partially rescued by the injection of *ythdf2* mRNA. Error bars, mean ± s.e.m., n = 8 embryos counted for each condition, 100 cells each embryo. P values were determined using unpaired Student’s t-test.

**Extended Data Figure 3. Characterization of m⁶A modification and gene expression change in the early embryonic transcriptome of zebrafish**
a, Venn diagrams show the overlap of two replicates of m⁶A-modified transcripts determined by m⁶A-seq and m⁶A-CLIP-seq at five time points. b, Distribution of m⁶A-sites (number of m⁶A-seq peaks) in the six gene groups over time. c, Number of m⁶A-modified genes identified in both m⁶A-seq and m⁶A-CLIP-seq at five time points. d, Quantification of the m⁶A/(G+A+C+U) ratio of mRNAs purified from zebrafish embryos at various developmental time points by LC-MS/MS. The fluctuation observed may reflect the complex dynamics of the decay of the methylated maternal transcripts, the transcription and methylation of newly synthesized zygotic mRNAs, and their subsequent decay during early development. All time points were normalized to 0 h.p.f. value. Error bars, mean ±s.d., n = 3 (technical replicates). e, Consensus motif identified by HOMER with m⁶A-CLIP peaks at five time points. f, Metagene profiles depict the subtranscript distribution pattern of m⁶A-sites (from m⁶A-seq) within the zebrafish transcriptome. m⁶A-seq peak signals are enriched after the start codon and before the stop codon. **g–h**, Gene Ontology (GO) analysis of non-methylated maternal transcripts **(g)** and methylated ones **(h). i**, Table showing the number of genes downregulated and upregulated in maternal *ythdf2^−/−* mutants at 4 h.p.f. within the 6 gene expression clusters (top 20%).

Extended Data Figure 4. Design and knockdown efficiency of the morpholino targeting *ythdf2*
a, Design of *ythdf2* morpholino that targets the intersection of the 5′ UTR and CDS of *ythdf2* mRNA. b, Efficiency test of *ythdf2* morpholino treatment inhibiting the expression of Ythdf2 by Western blot. mRNAs containing the 5′UTR and full-length CDS of *ythdf2* with FLAG tag sequence fused at the C-terminus were co-injected with either control or *ythdf2* MO into single cell wild-type embryos. Ythdf2-FLAG was detected by the anti-FLAG antibody. Material from six embryos was loaded into each lane. For gel source data, see Supplementary Figure 1.

**Extended Data Figure 5. Overlap between methylated genes and upregulated genes in maternal *ythdf2*^−/− embryos**
a, Quantification of the m⁶A/A ratio of the total mRNA purified from maternal *ythdf2*^−/− and wild-type embryos by LC-MS/MS. P values were determined using two-sided Student’s t-test for paired samples. Error bars, mean ± s.d. of two technical replicates (data points with same colour) from two biological experiments. b, Composition of upregulated, methylated genes in maternal *ythdf2*^−/− samples at 4 h.p.f. A total of 3,292 genes are both methylated and upregulated upon *ythdf2* LOF, over 60% of which are maternal genes. c, Venn diagram depicts the overlapping of m⁶A-modified transcripts determined by m⁶A-seq, m⁶A-CLIP-seq, and differentially expressed transcripts between *ythdf2* LOF samples and controls at 4 h.p.f. The 135 intersected genes were defined as the most stringent Ythdf2 RNA targets. Without including the MO-treated result (but with *ythdf2*^−/− result), we can estimate about 383 Ythdf2 target mRNAs. d, GO analysis of the most stringent Ythdf2 target genes.

**Extended Data Figure 6. Methylation profiles of Ythdf2 target transcripts**
a, Examples of Ythdf2 RNA targets harboring both m⁶A-seq peaks and m⁶A-CLIP-seq peaks. Coverage of m⁶A immunoprecipitation (IP) and input fragments are indicated in red and blue, respectively. m⁶A-CLIP-seq peaks are highlighted in green. Grey lines signify CDS borders. b, The dynamics of m⁶A immunoprecipitation (IP) signals over time in Ythdf2 RNA targets. Coverage of m⁶A immunoprecipitation (IP) and input fragments are indicated in red and grey, respectively. The locations of consensus motifs of GGACU and AGACU are indicated as blue dots. Red lines show splice junctions. Blue squares mark the peaks identified from the m⁶A-seq signals. Example genes shown: *buc*, *mtus1a*, *mylipa*, *setdb1a*, *tdrd1*, *vps26a*, and *zgc:162879*.

**Extended Data Figure 7. Degradation of reporter mRNAs in *ythdf2* LOF and control embryos**
The *in vivo* degradation of GFP-m⁶A mRNAs was faster than GFP-A mRNAs when co-injected with control MO. In contrast, GFP-m⁶A mRNAs displayed an undistinguishable decay rate from GFP-A mRNAs when co-injected with *ythdf2* MO. The abundances of GFP-m⁶A and GFP-A mRNAs were determined by RT-qPCR. Error bars, mean ± s.d, n = 3 (technical replicates). P values were determined using two-sided Student’s t-test for two samples with equal variance.

**Extended Data Figure 8. Zygotic Ythdf2 facilitates continued regulation of maternal mRNA clearance during late MZT (6–8 h.p.f.)**
Cumulative distribution of the log₂ fold changes of RNA expression after 4 h.p.f. for the six gene groups between maternal *ythdf2^−/−* (m-KO) and wild-type (**a, b**), or maternal-zygotic *ythdf2^−/−* (mz-KO) and wild-type (**c, d**). Colour code and gene numbers of each gene group are listed under each figure panel. Compared to the relatively unchanged curves of semi-stable gene groups (black and grey), the right shift of blue curves indicates the increase of maternal RNAs while the left shift of red curves indicates the decrease of zygotic RNAs in mutant samples versus wild-type ones. While the gene expression patterns of m-KO samples remain largely unchanged at 6 h.p.f. (a) and 8 h.p.f. (b), mz-KO samples show significant retention of maternal genes and delayed activation of zygotic genes at 6 h.p.f. (c) and continued notable delay of zygotic expression at 8 h.p.f. (d), indicating zygotically expressed Ythdf2 also functions on maternal mRNA during late MZT. Gene group categorization was based on the expression pattern data from 0 – 8 h.p.f. P values were calculated using two-sided Kruskal-Wallis test. Representative data shown from two independent experiments.

**Extended Data Figure 9. Ythdf2 does not cause transcriptome-wide zygotic gene expression change during the zygotic stage of zebrafish early development (12–48 h.p.f.)**
Cumulative distribution of the log₂ fold changes of RNA expression during the zygotic stage of zebrafish early development for the six gene groups between mutant and wild-type embryos. **a–b**, mz-KO samples exhibit largely unchanged gene expression patterns at 12 h.p.f. (a) and 24 h.p.f. (b) versus wild-type, indicating that Ythdf2 does not impact the degradation of zygotic mRNA on the organismal level during this time period. c, Considering the potential time-matched inaccuracy due to the delayed zygotic gene expression in the mutant embryos, we performed a stage-matched comparison at the 14–17 somite stage (WT 16 h.p.f. vs KO 17.5 h.p.f.) and observed very similar, unchanged gene expression profiles between mutant and wild-type embryos. Both time-matched and stage-matched comparisons reveal largely indistinguishable zygotic transcriptome profiles during the investigated zygotic stage corresponds nicely with the lack of abnormal developmental phenotypes in *ythdf2* mz-KO embryos during this time period. **d–f**, To avoid the involvement of paternal effect from male *ythdf2* KO fish, we used maternal KO embryos (from wild-type male) injected with *ythdf2*-MO (m-KO + MO) to achieve maternal and zygotic depletion of Ythdf2. We investigated the RNA expression of all genes between wild-type and *ythdf2* LOF groups during later developmental stages and observed largely unchanged gene expression patterns in m-KO + MO samples at 24 h.p.f. (d), 36 h.p.f. (e), and 48 h.p.f. (f) versus wild-type, which indicate that Ythdf2 does not impact the degradation of zygotic mRNA on the transcriptome level during late zygotic stages. Gene group categorization was based on the expression pattern data from 0 – 8 h.p.f. P values were calculated using two-sided Kruskal-Wallis test. Representative data shown from two independent experiments.

**Extended Data Figure 10. Target overlap of Ythdf2 and miR-430 regulation pathways**
a, Target overlap between Ythdf2 and miR-430. Genes with more than 1.5-fold upregulation upon removal of maternal Ythdf2 or miR-430 were used as corresponding targets in the analysis. b, GO analysis of the common target genes of Ythdf2 and miR-430. **c–f**, Differential temporal regulation of the targets of miR430 and Ythdf2. In wild-type embryos, common targets are decayed first, followed by Ythdf2-specific targets, and then miR430-specific targets (c). Upon maternal *ythdf2* knockout, the decay of the miR430-specific targets showed minimal delay (d), while the Ythdf2-specific targets and the common targets show more significant decay delays; the latter finding is consistent with Ythdf2 as a maternally-supplied factor able to degrade maternal mRNAs earlier in time (e, f). Relative expression level is calculated by the median of the expression levels of all genes and normalized to the value at 0 h.p.f., respectively. g, Maternal *ythdf2*^−/− embryos show a similar zygotic genome activation delay as Nanog+Sox19b+Pou5f3 LOF embryos at 4 h.p.f. The log₂ fold change of the mRNA-seq data of maternal *ythdf2*^−/− mutants and the published mRNA-seq data with combined loss of *nanog*, *sox19b* (*soxB1*), and *pou5f3* (*oct4* homolog) were plotted in the same diagram. Zygotic genes (red dots) were separately plotted out from all genes (grey dots). Intersections of crosslines mark the median points of each group of genes. h, proposed mechanism of Ythdf2 regulating zebrafish MZT: Ythdf2-mediated RNA decay ensures the timely clearance of maternal RNA; Without Ythdf2, m⁶A-modified maternal RNA are overrepresented in the transcriptome during MZT, causing delayed zygotic genome activation and a significant developmental delay.

**Figure 1. The deficiency of m⁶A-binding protein Ythdf2 in zebrafish embryo led to a developmental delay**
a, Expression profile of *ythdf2* mRNA in wild-type and maternal *ythdf2*^−/− embryos from crossing *ythdf2*^−/− female and wild-type male fish. mRNA-seq and *in situ* hybridization revealed the mutation eliminated *ythdf2* mRNA prior to 4 h.p.f. b, Crossing scheme to show the origin of maternal *ythdf2*^−/− embryos used in this study. c, Time-matched bright field images of embryos showing that maternal *ythdf2*^−/− embryos experience an early-stage delay maintained throughout early development. d, Quantification of DNA amounts in wild-type and mutant embryos pre- and post-MBT (2.5–5.5 h.p.f.). Error bars, mean ± s.e.m., n = 3 (biological replicates).

**Figure 2. Maternal RNAs are m⁶A-modified and their clearance is hindered by *ythdf2* loss-of-function (LOF)**
a, Expression profiles of six gene groups clustered by their RNA abundance over time (0 – 8 h.p.f.) determined by RNA sequencing: maternal-early (degradation starting at 2 h.p.f), maternal-late (decrease after 4 h.p.f.), zygotic-early (onset at 4 h.p.f. and peak at 6 h.p.f.), zygotic-late (onset at 6 h.p.f.), and two semi-stable gene groups (relatively stable expression over time). b, Distribution of m⁶A-sites (number of m⁶A-CLIP-seq peaks) in the six gene groups over time (the same colour code as a). c, Expression profiles of m⁶A-modified and non-modified maternal transcripts over time. **d–e**, Cumulative distribution of the log₂ fold changes of RNA expression at 4 h.p.f. for the six gene groups between maternal *ythdf2^−/−* and wild-type (d), or *ythdf2* morpholino (MO) treated and control (e). Compared to the relatively unchanged curves of semi-stable gene groups (black and grey), the right shift of blue curves indicates the increase of maternal RNAs while the left shift of red curves indicates the decrease of zygotic RNAs in *ythdf2* LOF samples versus control. P values were calculated using two-sided Kruskal-Wallis test. Representative data shown from two independent experiments.

**Figure 3. Ythdf2 is required for m⁶A-dependent RNA decay**
a, RT-qPCR validation of the relative abundance of representative Ythdf2 targets increased at 4 h.p.f. in maternal *ythdf2*^−/− samples compared with wild-type. b, Design of the reporter RNA. m⁶A-modified (GFP-m⁶A) or non-modified GFP mRNAs (GFP-A) were prepared and co-injected with mCherry control mRNA into one-cell stage embryos. c, The *in vivo* degradation of GFP-m⁶A mRNAs was faster in wild-type embryos but slightly slower in maternal *ythdf2*^−/− ones than GFP-A mRNAs. mRNA abundance was determined by RT-qPCR and normalized to 0.5 h.p.f. values. d, Comparison of GFP-m⁶A expression levels in wild-type and maternal *ythdf2*^−/− embryos at the oblong stage of development (stage-matched embryos, 3.8 h.p.f. for wild-type and 4.2 h.p.f. for mutants). The m⁶A-modified GFP reporter RNA produced consistently higher GFP protein levels in maternal *ythdf2*^−/− embryos versus wild-type embryos. Quantification of the overall phenomena: 61/72 embryos (85%) for maternal *ythdf2^−/−* and 59/85 (70%) for wild-type in two experiments. Error bars, s.d., n = 3 (technical replicates). P values were determined using two-sided Student’s t-test for two samples with equal variance.

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Comment in

RNA Methylation Clears the Way.
Kontur C, Giraldez A. Kontur C, et al. Dev Cell. 2017 Mar 13;40(5):427-428. doi: 10.1016/j.devcel.2017.02.024. Dev Cell. 2017. PMID: 28292421
Mark it for destruction: a novel role of mRNA methylation in maternal-to-zygotic transition†.
Yan W. Yan W. Biol Reprod. 2017 Apr 1;96(4):829-830. doi: 10.1093/biolre/iox016. Biol Reprod. 2017. PMID: 28371885 Free PMC article. No abstract available.

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References

1. Tadros W, Lipshitz HD. The maternal-to-zygotic transition: a play in two acts. Development. 2009;136:3033–3042. - PubMed
1. Lee MT, Bonneau AR, Giraldez AJ. Zygotic genome activation during the maternal-to-zygotic transition. Annu Rev Cell Dev Bi. 2014;30:581–613. - PMC - PubMed
1. Walser CB, Lipshitz HD. Transcript clearance during the maternal-to-zygotic transition. Curr Opin Genet Dev. 2011;21:431–443. - PubMed
1. Giraldez AJ, et al. Zebrafish miR-430 promotes deadenylation and clearance of maternal mRNAs. Science. 2006;312:75–79. - PubMed
1. Mishima Y, Tomari Y. Codon usage and 3′UTR length determine maternal mRNA stability in zebrafish. Mol Cell. 2016;61:874–885. - PubMed

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[1] Tadros W, Lipshitz HD. The maternal-to-zygotic transition: a play in two acts. Development. 2009;136:3033–3042. - PubMed

[2] Tadros W, Lipshitz HD. The maternal-to-zygotic transition: a play in two acts. Development. 2009;136:3033–3042. - PubMed

[3] Lee MT, Bonneau AR, Giraldez AJ. Zygotic genome activation during the maternal-to-zygotic transition. Annu Rev Cell Dev Bi. 2014;30:581–613. - PMC - PubMed

[4] Lee MT, Bonneau AR, Giraldez AJ. Zygotic genome activation during the maternal-to-zygotic transition. Annu Rev Cell Dev Bi. 2014;30:581–613. - PMC - PubMed

[5] Walser CB, Lipshitz HD. Transcript clearance during the maternal-to-zygotic transition. Curr Opin Genet Dev. 2011;21:431–443. - PubMed

[6] Walser CB, Lipshitz HD. Transcript clearance during the maternal-to-zygotic transition. Curr Opin Genet Dev. 2011;21:431–443. - PubMed

[7] Giraldez AJ, et al. Zebrafish miR-430 promotes deadenylation and clearance of maternal mRNAs. Science. 2006;312:75–79. - PubMed

[8] Giraldez AJ, et al. Zebrafish miR-430 promotes deadenylation and clearance of maternal mRNAs. Science. 2006;312:75–79. - PubMed

[9] Mishima Y, Tomari Y. Codon usage and 3′UTR length determine maternal mRNA stability in zebrafish. Mol Cell. 2016;61:874–885. - PubMed

[10] Mishima Y, Tomari Y. Codon usage and 3′UTR length determine maternal mRNA stability in zebrafish. Mol Cell. 2016;61:874–885. - PubMed

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m⁶A-dependent maternal mRNA clearance facilitates zebrafish maternal-to-zygotic transition

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m⁶A-dependent maternal mRNA clearance facilitates zebrafish maternal-to-zygotic transition

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