doi: 10.1016/0014-4827(89)90347-9.
Specificity and timing of the Zn2+ requirement for DNA synthesis by 3T3 cells
Affiliations
- PMID: 2806401
- DOI: 10.1016/0014-4827(89)90347-9
Item in Clipboard
Specificity and timing of the Zn2+ requirement for DNA synthesis by 3T3 cells
Exp Cell Res.
1989 Oct.
Abstract
Addition of the chelator DTPA to synchronized cultures of 3T3 cells inhibited thymidine incorporation by up to 90% and only Zn2+ of the divalent cations tested was effective in reversing this effect. Fe2+ given alone had no effect on the inhibition by DTPA but when added to Zn2+ supplemented cultures increased thymidine incorporation from approximately 80-90 to over 100% of that in control cultures. Investigations indicated that the major requirement for Zn2+ was within the period from 8 h after stimulation of quiescent cells with serum until 3 h before the start of S phase. There was also an indication of a further requirement for Zn2+ at the G/S transition.
Similar articles
-
Zinc deprivation of murine 3T3 cells by use of diethylenetrinitrilopentaacetate impairs DNA synthesis upon stimulation with insulin-like growth factor-1 (IGF-1).J Nutr. 1998 Oct;128(10):1600-5. doi: 10.1093/jn/128.10.1600. J Nutr. 1998. PMID: 9772124
-
Ca2+ and Zn2+ dependence of DNA synthesis in untransformed and in Ha-ras(val-12)-expressing NIH 3T3 cells.Exp Cell Res. 1993 Sep;208(1):303-10. doi: 10.1006/excr.1993.1250. Exp Cell Res. 1993. PMID: 8359224
-
Thymidine incorporation is less sensitive to lack of zinc in human than in rodent cells.Biol Trace Elem Res. 1995 Aug-Sep;49(2-3):119-27. doi: 10.1007/BF02788961. Biol Trace Elem Res. 1995. PMID: 8562280
-
Mechanism of action of the inhibitory effect of nifedipine on the growth of cultured aortic cells from spontaneously hypertensive and normotensive rats.Br J Pharmacol. 1995 Apr;114(8):1703-9. doi: 10.1111/j.1476-5381.1995.tb14960.x. Br J Pharmacol. 1995. PMID: 7541285 Free PMC article.
-
Ethanol has multiple effects on DNA synthesis in fibroblasts depending on the presence of secreted growth regulators and zinc as well as the level of protein kinase C activation.Arch Biochem Biophys. 2000 Sep 1;381(1):127-34. doi: 10.1006/abbi.2000.1954. Arch Biochem Biophys. 2000. PMID: 11019828
Cited by
-
Role of the promoter in the sensitivity of human thymidine kinase to lack of Zn2+.Biochem J. 1995 Jun 1;308 ( Pt 2)(Pt 2):659-64. doi: 10.1042/bj3080659. Biochem J. 1995. PMID: 7772056 Free PMC article.
-
Zinc is required for the expression of ornithine decarboxylase in a difluoromethylornithine-resistant cell line.Biochem J. 1994 Apr 15;299 ( Pt 2)(Pt 2):515-9. doi: 10.1042/bj2990515. Biochem J. 1994. PMID: 8172613 Free PMC article.
-
A requirement for Zn2+ for the induction of thymidine kinase but not ornithine decarboxylase in 3T3 cells stimulated from quiescence.Biochem J. 1990 Dec 1;272(2):525-7. doi: 10.1042/bj2720525. Biochem J. 1990. PMID: 2268279 Free PMC article.
-
The biology of zinc transport in mammary epithelial cells: implications for mammary gland development, lactation, and involution.J Mammary Gland Biol Neoplasia. 2014 Mar;19(1):59-71. doi: 10.1007/s10911-013-9314-4. Epub 2013 Dec 15. J Mammary Gland Biol Neoplasia. 2014. PMID: 24338187 Review.
-
The Crossroads between Zinc and Steroidal Implant-Induced Growth of Beef Cattle.Animals (Basel). 2021 Jun 27;11(7):1914. doi: 10.3390/ani11071914. Animals (Basel). 2021. PMID: 34199133 Free PMC article. Review.