Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1989 Oct 11;17(19):7905-21.
doi: 10.1093/nar/17.19.7905.

Nucleotide substitutions within the cardiac troponin T alternative exon disrupt pre-mRNA alternative splicing

T A Cooper  1 , C P Ordahl
Affiliations
Free PMC article

Nucleotide substitutions within the cardiac troponin T alternative exon disrupt pre-mRNA alternative splicing

T A Cooper et al. Nucleic Acids Res. .
Free PMC article

Abstract

The cardiac troponin T (cTNT) pre-mRNA contains a single alternative exon (exon 5) which is either included or excluded from the processed mRNA. Using transient transfection of cTNT minigenes, we have previously localized pre-mRNA cis elements required for exon 5 alternative splicing to three small regions of the pre-mRNA which include exons 4, 5, and 6. In the present study, nucleotide substitutions were introduced into the region containing exon 5 to begin to define specific nucleotides required for exon 5 alternative splicing. A mutation within the 5' splice site flanking the cTNT alternative exon that increases its homology to the consensus sequence improves splicing efficiency and leads to increased levels of mRNAs that include the alternative exon. Surprisingly, substitution of as few as four nucleotides within the alternative exon disrupts cTNT pre-mRNA alternative splicing and prevents recognition of exon 5 as a bona fide exon. These results establish that the cTNT alternative exon contains information in cis that is required for its recognition by the splicing machinery.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Proc Natl Acad Sci U S A. 1987 Nov;84(22):7928-32 - PubMed
    1. J Virol. 1987 Dec;61(12):3938-45 - PubMed
    1. Cell. 1988 Jan 29;52(2):207-19 - PubMed
    1. Mol Cell Biol. 1987 Dec;7(12):4297-307 - PubMed
    1. Mol Cell Biol. 1988 Feb;8(2):860-6 - PubMed

Publication types