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. 2016 Nov 10:22:4283-4288.
doi: 10.12659/MSM.897315.

Allyl Isothiocyanate Inhibits the Proliferation of Renal Carcinoma Cell Line GRC-1 by Inducing an Imbalance Between Bcl2 and Bax

Zhongyong Jiang  1 , Xi Liu  2 , Kai Chang  1 , Xia Liu  1 , Jie Xiong  1
Affiliations

Allyl Isothiocyanate Inhibits the Proliferation of Renal Carcinoma Cell Line GRC-1 by Inducing an Imbalance Between Bcl2 and Bax

Zhongyong Jiang et al. Med Sci Monit. .

Abstract

BACKGROUND Because of the insensitivity of renal cell carcinoma (RCC) to both chemotherapy and radiotherapy, surgery remains the primary approach for anticancer treatment. However, patients who do not receive timely diagnoses may not be suitable for surgery, especially in the late phase of tumor development. Thus, the discovery of novel effective treatment is of great importance. Allyl isothiocyanate (AITC) can inhibit the proliferation and induce apoptosis in many cancer cells. In this paper, we report on an in vitro study to determine the effect of AITC on proliferation and apoptosis of RCC line GRC-1. MATERIAL AND METHODS CCK8 assay was used to detect cell proliferation under gradient concentrations of AITC. Flow cytometry was employed to evaluate cell apoptosis. Real-time fluorescent polymerase chain reaction quantified mRNA levels of Bax and Bcl-2 genes. Western blotting was further employed for protein expression assay. RESULTS AITC inhibited GRC-1 cell proliferation and induced cell apoptosis in a dose-dependent manner; it also elevated Bax while suppressing Bcl-2 gene expression at both mRNA and protein levels. In general, increasing concentration of AITC decreased Bcl-2/Bax ratio. CONCLUSIONS The inhibitory effect of AITC on GRC-1 cells is exerted via cell apoptosis, in which the imbalance of Bcl-2/Bax plays a significant role.

Keywords: Alprenolol; Apoptosis Inducing Factor; Cellulose 1,4-beta-Cellobiosidase.

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Conflict of interest statement

of conflict of interest The authors declare no competing financial or commercial interests in this manuscript.

Figures

Figure 1
Figure 1
Cell apoptosis under allyl isothiocyanate.
Figure 2
Figure 2
Bax and Bcl-2 mRNA levels. * P<0.05, compared with 0-μM group.
Figure 3
Figure 3
Bax and Bcl-2 protein expression levels.
See this image and copyright information in PMC

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