doi: 10.1016/0006-291x(89)92111-6.
Prenylated proteins: demonstration of a thioether linkage to cysteine of proteins
Affiliations
- PMID: 2775255
- DOI: 10.1016/0006-291x(89)92111-6
Item in Clipboard
Prenylated proteins: demonstration of a thioether linkage to cysteine of proteins
Biochem Biophys Res Commun.
.
Abstract
Prenylated amino acid fragments have been isolated from prenylated proteins of Chinese hamster ovary cells. Gel-exclusion chromatography indicates that these proteins are modified by two different prenyl groups. The prenyl-amino acid fragments are labeled by 35S from cysteine, and this bond is cleaved by Raney-Ni, proving that the prenyl residue is linked to protein via a thioether to cysteine. Hydrazinolysis has been used to demonstrate that the cysteine is carboxy terminal.
Similar articles
-
Quantitation of prenylcysteines by a selective cleavage reaction.Proc Natl Acad Sci U S A. 1991 Nov 1;88(21):9668-70. doi: 10.1073/pnas.88.21.9668. Proc Natl Acad Sci U S A. 1991. PMID: 1946384 Free PMC article.
-
Prenylated proteins: the structure of the isoprenoid group.Science. 1990 Jan 19;247(4940):318-20. doi: 10.1126/science.2296720. Science. 1990. PMID: 2296720
-
Isopentenoid synthesis in embryonic Drosophila cells: prenylated protein profile and prenyl group usage.Arch Biochem Biophys. 1992 Jun;295(2):410-20. doi: 10.1016/0003-9861(92)90535-5. Arch Biochem Biophys. 1992. PMID: 1586169
-
The prenylation of proteins.Bioessays. 1992 Jan;14(1):25-31. doi: 10.1002/bies.950140106. Bioessays. 1992. PMID: 1546978 Review.
-
[Successes in studying the physiological activity of terpenoids and steroids].Biokhimiia. 1992 Jul;57(7):986-1003. Biokhimiia. 1992. PMID: 1391217 Review. Russian.
Cited by
-
Quantitation of prenylcysteines by a selective cleavage reaction.Proc Natl Acad Sci U S A. 1991 Nov 1;88(21):9668-70. doi: 10.1073/pnas.88.21.9668. Proc Natl Acad Sci U S A. 1991. PMID: 1946384 Free PMC article.
-
Purification and characterization of recombinant human farnesyl diphosphate synthase expressed in Escherichia coli.Biochem J. 1991 Apr 1;275 ( Pt 1)(Pt 1):61-5. doi: 10.1042/bj2750061. Biochem J. 1991. PMID: 2018485 Free PMC article.
-
Prenylated proteins: synthesis of geranylgeranylcysteine and identification of this thioether amino acid as a component of proteins in CHO cells.Proc Natl Acad Sci U S A. 1990 Oct;87(19):7352-4. doi: 10.1073/pnas.87.19.7352. Proc Natl Acad Sci U S A. 1990. PMID: 2217168 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
