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. 1989 Aug;86(15):5979-82.
doi: 10.1073/pnas.86.15.5979.

Characterization of a RNA virus from the parasite Leishmania

G Widmer  1 , A M Comeau, D B Furlong, D F Wirth, J L Patterson
Affiliations

Characterization of a RNA virus from the parasite Leishmania

G Widmer et al. Proc Natl Acad Sci U S A. 1989 Aug.

Abstract

We were interested in screening a series of isolates of the protozoan Leishmania for the presence of viruses. The experimental procedure we used was based on an enzymatic assay originally developed for viral RNA-dependent RNA polymerases. Simultaneously, total promastigote nucleic acid preparations were analyzed for the presence of viral genome and/or transcripts. Two isolates, both classified as L. braziliensis guyanensis, were found to be positive for RNA polymerase activity and to carry a large (6 kilobases) RNA species. The polymerase reaction products hybridized to the 6-kilobase RNA, believed to be the viral genome. In conjunction with electron microscopical observations these results indicate the presence of an RNA virus in these Leishmania isolates.

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