Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2016 Oct;11(10):1833-50.
doi: 10.1038/nprot.2016.110. Epub 2016 Sep 1.

Generation of human muscle fibers and satellite-like cells from human pluripotent stem cells in vitro

Jérome Chal  1   2   3   4 , Ziad Al Tanoury  1 , Marie Hestin  2   3   4 , Bénédicte Gobert  1 , Suvi Aivio  2   3   4 , Aurore Hick  5 , Thomas Cherrier  1 , Alexander P Nesmith  6 , Kevin K Parker  6 , Olivier Pourquié  1   2   3   4
Affiliations
Free article

Generation of human muscle fibers and satellite-like cells from human pluripotent stem cells in vitro

Jérome Chal et al. Nat Protoc. 2016 Oct.
Free article

Abstract

Progress toward finding a cure for muscle diseases has been slow because of the absence of relevant cellular models and the lack of a reliable source of muscle progenitors for biomedical investigation. Here we report an optimized serum-free differentiation protocol to efficiently produce striated, millimeter-long muscle fibers together with satellite-like cells from human pluripotent stem cells (hPSCs) in vitro. By mimicking key signaling events leading to muscle formation in the embryo, in particular the dual modulation of Wnt and bone morphogenetic protein (BMP) pathway signaling, this directed differentiation protocol avoids the requirement for genetic modifications or cell sorting. Robust myogenesis can be achieved in vitro within 1 month by personnel experienced in hPSC culture. The differentiating culture can be subcultured to produce large amounts of myogenic progenitors amenable to numerous downstream applications. Beyond the study of myogenesis, this differentiation method offers an attractive platform for the development of relevant in vitro models of muscle dystrophies and drug screening strategies, as well as providing a source of cells for tissue engineering and cell therapy approaches.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Front Cell Dev Biol. 2015 May 13;3:29 - PubMed
    1. Nat Protoc. 2006;1(3):1092-104 - PubMed
    1. C R Acad Sci III. 1995 Jan;318(1):57-66 - PubMed
    1. Dev Biol. 1986 Sep;117(1):319-25 - PubMed
    1. Toxicol In Vitro. 2001 Dec;15(6):741-53 - PubMed

Publication types