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. 2017 Mar;31(2):e22033.
doi: 10.1002/jcla.22033. Epub 2016 Aug 8.

The ACE2 G8790A Polymorphism: Involvement in Type 2 Diabetes Mellitus Combined with Cerebral Stroke

Yan-Hui Wu  1 , Jia-Ying Li  1 , Chi Wang  1 , Li-Mei Zhang  1 , Hong Qiao  1
Affiliations

The ACE2 G8790A Polymorphism: Involvement in Type 2 Diabetes Mellitus Combined with Cerebral Stroke

Yan-Hui Wu et al. J Clin Lab Anal. 2017 Mar.

Abstract

Background: We aimed to investigate the correlations between ACE2 polymorphisms and type 2 diabetes mellitus (T2DM) combined with cerebral stroke (CS).

Methods: A total of 346 patients treated or hospitalized in our hospital were enrolled, including 181 cases without cerebrovascular complications (T2DM group) and 165 cases combined with CS (T2DM + CS group); 284 healthy individuals were selected as the control group. PCR-RFLP and ELISA were used to analyze ACE2 G8790A polymorphisms and serum ACE2 levels, respectively.

Results: Significant differences were observed in the genotype/allele frequency of ACE2 G8790A between the T2DM + CS and control groups, and the T2DM and T2DM + CS groups, and in the genotype frequency of ACE2 G8790A between the T2DM and the control groups. The A allele may increase the risk of T2DM combined with CS. The AA genotype may also increase the risk of T2DM combined with CS (OR = 3.733, 95%CI = 2.069-6.738; OR = 3.597, 95%CI = 1.884-6.867). Serum ACE2 levels showed statistically significant differences among the groups. Systolic pressure and diastolic pressure were protective factors of T2DM combined with CS.

Conclusion: The ACE2 G8790A polymorphism in T2DM patients was correlated with CS, and the A allele might be a risk factor of T2DM combined with CS.

Keywords: ACE2; allele frequency; cerebral stroke; gene polymorphism; regression analysis; type 2 diabetes mellitus.

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Figures

Figure 1
Figure 1
Sequencing of the ACE2 G8790A polymorphism (ACE2: angiotensin‐converting enzyme 2; (a) the sequencing result of the ACE2 gene containing the A allele; (b) the sequencing result of the ACE2 gene containing the G allele; (c) the sequencing result of the ACE2 gene containing [C/T] heterozygotes; ▲: polymorphic site).
Figure 2
Figure 2
Electrophoresis of ACE2 gene G8709A polymorphism by enzyme digestion (ACE2: angiotensin‐converting enzyme 2; M: marker).
See this image and copyright information in PMC

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References

    1. Lin Y, Sun Z. Current views on type 2 diabetes. J Endocrinol 2010;204:1–11. - PMC - PubMed
    1. Chen L, Magliano DJ, Zimmet PZ. The worldwide epidemiology of type 2 diabetes mellitus – present and future perspectives. Nat Rev Endocrinol 2012;8:228–236. - PubMed
    1. Chan JC, Malik V, Jia W, et al. Diabetes in Asia: epidemiology, risk factors, and pathophysiology. JAMA 2009;301:2129–2140. - PubMed
    1. Yang SH, Dou KF, Song WJ. Prevalence of diabetes among men and women in China. N Engl J Med 2010;362:2425–2426; author reply 2426. - PubMed
    1. SEARCH for Diabetes in Youth Study Group , Liese AD, D'Agostino RB Jr, et al. The burden of diabetes mellitus among US youth: prevalence estimates from the SEARCH for Diabetes in Youth Study. Pediatrics 2006;118:1510–1518. - PubMed

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