Respiratory Syncytial Virus (RSV): Neutralizing Antibody, a Correlate of Immune Protection

doi:10.1007/978-1-4939-3687-8_7

. 2016:1442:77-91.

doi: 10.1007/978-1-4939-3687-8_7.

Respiratory Syncytial Virus (RSV): Neutralizing Antibody, a Correlate of Immune Protection

Pedro A Piedra^{1

2

3}, Anne M Hause^{4

5}, Letisha Aideyan⁴

Affiliations

¹ Department of Molecular Virology and Microbiology, Baylor College of Medicine, BCM MS280, One Baylor Plaza, Houston, TX, 77030, USA. ppiedra@bcm.edu.
² Department of Pediatrics, Baylor College of Medicine, Houston, TX, USA. ppiedra@bcm.edu.
³ Translational Biology and Molecular Medicine, Baylor College of Medicine, Houston, TX, USA. ppiedra@bcm.edu.
⁴ Department of Molecular Virology and Microbiology, Baylor College of Medicine, BCM MS280, One Baylor Plaza, Houston, TX, 77030, USA.
⁵ Translational Biology and Molecular Medicine, Baylor College of Medicine, Houston, TX, USA.

PMID: 27464689
DOI: 10.1007/978-1-4939-3687-8_7

Respiratory Syncytial Virus (RSV): Neutralizing Antibody, a Correlate of Immune Protection

Pedro A Piedra et al. Methods Mol Biol. 2016.

. 2016:1442:77-91.

doi: 10.1007/978-1-4939-3687-8_7.

Authors

Pedro A Piedra^{1

2

3}, Anne M Hause^{4

5}, Letisha Aideyan⁴

Affiliations

¹ Department of Molecular Virology and Microbiology, Baylor College of Medicine, BCM MS280, One Baylor Plaza, Houston, TX, 77030, USA. ppiedra@bcm.edu.
² Department of Pediatrics, Baylor College of Medicine, Houston, TX, USA. ppiedra@bcm.edu.
³ Translational Biology and Molecular Medicine, Baylor College of Medicine, Houston, TX, USA. ppiedra@bcm.edu.
⁴ Department of Molecular Virology and Microbiology, Baylor College of Medicine, BCM MS280, One Baylor Plaza, Houston, TX, 77030, USA.
⁵ Translational Biology and Molecular Medicine, Baylor College of Medicine, Houston, TX, USA.

PMID: 27464689
DOI: 10.1007/978-1-4939-3687-8_7

Abstract

Assays that measure RSV-specific neutralizing antibody activity are very useful for evaluating vaccine candidates, performing seroprevalence studies, and detecting infection. Neutralizing antibody activity is normally measured by a plaque reduction neutralization assay or by a microneutralization assay with or without complement. These assays measure the functional capacity of serum (or other fluids) to neutralize virus infectivity in cells as compared to ELISA assays that only measure the binding capacity against an antigen. This chapter discusses important elements in standardization of the RSV-specific microneutralization assay for use in the laboratory.

Keywords: Microneutralization assay; Neutralization assay; Neutralizing antibodies.

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Respiratory Syncytial Virus (RSV): Neutralizing Antibody, a Correlate of Immune Protection

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Respiratory Syncytial Virus (RSV): Neutralizing Antibody, a Correlate of Immune Protection

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