Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1989 Jun;86(11):3978-81.
doi: 10.1073/pnas.86.11.3978.

Human basic fibroblast growth factor gene encodes four polypeptides: three initiate translation from non-AUG codons

R Z Florkiewicz  1 , A Sommer
Affiliations

Human basic fibroblast growth factor gene encodes four polypeptides: three initiate translation from non-AUG codons

R Z Florkiewicz et al. Proc Natl Acad Sci U S A. 1989 Jun.

Erratum in

  • Proc Natl Acad Sci U S A 1990 Mar;87(5):2045

Abstract

Human basic fibroblast growth factor (bFGF) is an angiogenic polypeptide mitogen present in a wide variety of mesoderm- and neuroectoderm-derived tissues. bFGF cDNA and genomic clones predict a 17.8-kDa (155-amino acid) gene product based on the presence of a single putative translational initiator ATG codon. However, a bFGF protein isolated from human placenta contains two additional amino acids NH2-terminal to the predicted initiator methionine. We report here that the human cell line SK-HEP-1 coexpresses four molecular forms (17.8, 22.5, 23.1, and 24.2 kDa) of bFGF. The 17.8-kDa bFGF protein is translationally initiated at the previously predicted methionine (AUG) codon, whereas the 22.5-, 23.1-, and 24.2-kDa proteins initiate at unusual non-AUG codons. The higher molecular weight forms are colinear NH2-terminal extensions of the 18-kDa bFGF.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. J Cell Biol. 1983 Nov;97(5 Pt 1):1381-8 - PubMed
    1. J Biol Chem. 1988 Nov 5;263(31):16297-302 - PubMed
    1. Proc Natl Acad Sci U S A. 1984 Apr;81(7):2050-4 - PubMed
    1. Cell. 1986 Jan 31;44(2):283-92 - PubMed
    1. Proc Natl Acad Sci U S A. 1986 Apr;83(7):2091-5 - PubMed