Transforming properties of Felis catus papillomavirus type 2 E6 and E7 putative oncogenes in vitro and their transcriptional activity in feline squamous cell carcinoma in vivo

doi:10.1016/j.virol.2016.05.017

. 2016 Sep:496:1-8.

doi: 10.1016/j.virol.2016.05.017. Epub 2016 May 26.

Transforming properties of Felis catus papillomavirus type 2 E6 and E7 putative oncogenes in vitro and their transcriptional activity in feline squamous cell carcinoma in vivo

Gennaro Altamura¹, Annunziata Corteggio², Laura Pacini³, Andrea Conte⁴, Giovanna Maria Pierantoni⁵, Massimo Tommasino⁶, Rosita Accardi⁷, Giuseppe Borzacchiello⁸

Affiliations

¹ Department of Veterinary Medicine and Animal Productions, General Pathology and Pathological Anatomy Unit, University of Naples Federico II, Via Delpino 1, 80137 Naples, Italy. Electronic address: gennaro.altamura@unina.it.
² Department of Veterinary Medicine and Animal Productions, General Pathology and Pathological Anatomy Unit, University of Naples Federico II, Via Delpino 1, 80137 Naples, Italy. Electronic address: ancorteg@unina.it.
³ Infections and Cancer Biology Group, International Agency for Research on Cancer, 150 Cours Albert Thomas, 69372 Lyon, France. Electronic address: PaciniL@students.iarc.fr.
⁴ Department of Molecular Medicine and Medical Biotechnologies, University of Naples Federico II, Via Pansini 5, 80131 Naples, Italy. Electronic address: andreaconte88@hotmail.it.
⁵ Department of Molecular Medicine and Medical Biotechnologies, University of Naples Federico II, Via Pansini 5, 80131 Naples, Italy. Electronic address: gmpieran@unina.it.
⁶ Infections and Cancer Biology Group, International Agency for Research on Cancer, 150 Cours Albert Thomas, 69372 Lyon, France. Electronic address: tommasinom@iarc.fr.
⁷ Infections and Cancer Biology Group, International Agency for Research on Cancer, 150 Cours Albert Thomas, 69372 Lyon, France. Electronic address: accardir@iarc.fr.
⁸ Department of Veterinary Medicine and Animal Productions, General Pathology and Pathological Anatomy Unit, University of Naples Federico II, Via Delpino 1, 80137 Naples, Italy. Electronic address: borzacch@unina.it.

PMID: 27236740
DOI: 10.1016/j.virol.2016.05.017

Free article

Transforming properties of Felis catus papillomavirus type 2 E6 and E7 putative oncogenes in vitro and their transcriptional activity in feline squamous cell carcinoma in vivo

Gennaro Altamura et al. Virology. 2016 Sep.

Free article

. 2016 Sep:496:1-8.

doi: 10.1016/j.virol.2016.05.017. Epub 2016 May 26.

Authors

Gennaro Altamura¹, Annunziata Corteggio², Laura Pacini³, Andrea Conte⁴, Giovanna Maria Pierantoni⁵, Massimo Tommasino⁶, Rosita Accardi⁷, Giuseppe Borzacchiello⁸

Affiliations

¹ Department of Veterinary Medicine and Animal Productions, General Pathology and Pathological Anatomy Unit, University of Naples Federico II, Via Delpino 1, 80137 Naples, Italy. Electronic address: gennaro.altamura@unina.it.
² Department of Veterinary Medicine and Animal Productions, General Pathology and Pathological Anatomy Unit, University of Naples Federico II, Via Delpino 1, 80137 Naples, Italy. Electronic address: ancorteg@unina.it.
³ Infections and Cancer Biology Group, International Agency for Research on Cancer, 150 Cours Albert Thomas, 69372 Lyon, France. Electronic address: PaciniL@students.iarc.fr.
⁴ Department of Molecular Medicine and Medical Biotechnologies, University of Naples Federico II, Via Pansini 5, 80131 Naples, Italy. Electronic address: andreaconte88@hotmail.it.
⁵ Department of Molecular Medicine and Medical Biotechnologies, University of Naples Federico II, Via Pansini 5, 80131 Naples, Italy. Electronic address: gmpieran@unina.it.
⁶ Infections and Cancer Biology Group, International Agency for Research on Cancer, 150 Cours Albert Thomas, 69372 Lyon, France. Electronic address: tommasinom@iarc.fr.
⁷ Infections and Cancer Biology Group, International Agency for Research on Cancer, 150 Cours Albert Thomas, 69372 Lyon, France. Electronic address: accardir@iarc.fr.
⁸ Department of Veterinary Medicine and Animal Productions, General Pathology and Pathological Anatomy Unit, University of Naples Federico II, Via Delpino 1, 80137 Naples, Italy. Electronic address: borzacch@unina.it.

PMID: 27236740
DOI: 10.1016/j.virol.2016.05.017

Abstract

Felis catus papillomavirus type 2 (FcaPV2) DNA is found in feline cutaneous squamous cell carcinomas (SCCs); however, its biological properties are still uncharacterized. In this study, we successfully expressed FcaPV2 E6 and E7 putative oncogenes in feline epithelial cells and demonstrated that FcaPV2 E6 binds to p53, impairing its protein level. In addition, E6 and E7 inhibited ultraviolet B (UVB)-triggered accumulation of p53, p21 and pro-apoptotic markers such as Cleaved Caspase3, Bax and Bak, suggesting a synergistic action of the virus with UV exposure in tumour pathogenesis. Furthermore, FcaPV2 E7 bound to feline pRb and impaired pRb levels, resulting in upregulation of the downstream pro-proliferative genes Cyclin A and Cdc2. Importantly, we demonstrated mRNA expression of FcaPV2 E2, E6 and E7 in feline SCC samples, strengthening the hypothesis of a causative role in the development of feline SCC.

Keywords: E6; E7; Feline SCC; Feline oncology; Felis catus papillomavirus; Oncogenic viruses; Veterinary medicine; Veterinary virology.

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Transforming properties of Felis catus papillomavirus type 2 E6 and E7 putative oncogenes in vitro and their transcriptional activity in feline squamous cell carcinoma in vivo

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Transforming properties of Felis catus papillomavirus type 2 E6 and E7 putative oncogenes in vitro and their transcriptional activity in feline squamous cell carcinoma in vivo

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