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. 1989 Jul;53(1):226-33.
doi: 10.1111/j.1471-4159.1989.tb07318.x.

Muscarinic agonists cause calcium influx and calcium mobilization in forebrain neurons in vitro

I J Reynolds  1 , R J Miller
Affiliations

Muscarinic agonists cause calcium influx and calcium mobilization in forebrain neurons in vitro

I J Reynolds et al. J Neurochem. 1989 Jul.

Abstract

We have examined the effects of the muscarinic agonist carbachol on the intracellular free Ca2+ concentration ([Ca2+]i) in primary cultures of neurons from rat forebrain using the Ca2+-sensitive fluorescent dye fura-2. Addition of carbachol increased the [Ca2+]i in approximately 60% of cells studied. Oxotremorine-M, but not pilocarpine, mimicked the effects of carbachol. The response was reduced by 60% on removal of extracellular Ca2+, a finding suggesting that muscarinic receptor activation causes Ca2+ influx in addition to intracellular Ca2+ mobilization. Tetrodotoxin and nitrendipine also significantly reduced the response to carbachol. These studies suggest that the changes in [Ca2+]i produced by activation of muscarinic receptors result in part from mobilization of intracellular Ca2+ and that influx through voltage-sensitive Ca2+ channels also provides a significant contribution to the net [Ca2+]i change observed.

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