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. 2016 Jun;7(6):450-4.
doi: 10.1007/s13238-016-0275-4.

The crystal structure of Zika virus helicase: basis for antiviral drug design

Hongliang Tian  1   2 , Xiaoyun Ji  3 , Xiaoyun Yang  1 , Wei Xie  1 , Kailin Yang  4 , Cheng Chen  1 , Chen Wu  1 , Heng Chi  1 , Zhongyu Mu  1 , Zefang Wang  1 , Haitao Yang  5   6
Affiliations

The crystal structure of Zika virus helicase: basis for antiviral drug design

Hongliang Tian et al. Protein Cell. 2016 Jun.
No abstract available

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Figures

Fig. 1
Fig. 1
The monomeric structure of ZIKV helicase. (A) Size-exclusion chromatograms of ZIKV helicase. The molecular masses of protein standards are indicated at the top. (B) The overall structure of ZIKV helicase with the three domains colored and labeled respectively. (C) A cartoon diagram illustrating of the overall fold with potential RNA binding site and NTPase active site labelled. (D) Structure-based phylogenetic tree of 8 viral helicase structures from the Flaviviradae family using the program SHP (Stuart et al., 1979) and PHYLIP (Felsenstein, 1997). The following structures with PDB ID in parentheses are included: DENV-2 (2BMF), DENV-4 (2JLQ), JEV (2Z83), KUNV (2QEQ), YFV (1YKS), MVEV (2V8O), HCV (1HEI)
Fig. 2
Fig. 2
Structural insight into ZIKV helicase. (A) Cartoon and surface representation of the overall fold with the three domains of ZIKV helicase, colored and labeled respectively; (B) The electrostatic surface representation showing the tunnel for potential RNA binding. Positive potentials are colored blue and the negative are colored red. The putative position of the nucleic acid is marked as semi-transparent sticks. The model was obtained by superposition with the DENV-4 helicase in complex with ssRNA (PDB code 2JLV). (C) A clear view of the NTPase active site. The positions of putative nucleotide substrate (as sticks) and Mn2+ (as sphere) are marked semi-transparently by superposition with the DENV-4 helicase bound to AMPPNP and Mn2+ (PDB code 2JLR). P-loop is shown in red. (D) Isolated  P-loops are shown by superimposing the structures of 7 flavivirus apo helicases. ZIKV helicase is in red ribbon and the others are shown in finer lines. The P-loop of DENV-4 helicase is colored green. The following structures of helicases with PDB ID in parentheses are included: DENV-2 (2BMF), DENV-4 (2JLQ), JEV (2Z83), KUNV (2QEQ), YFV (1YKS), MVEV (2V8O). (E) Interactions at NTPase active site by superposition of ZIKV helicase (solid) with DENV-4 helicase in complex with AMPPNP and Mn2+ (semitransparent, PDB code 2JLR). Conserved residues are shown as sticks and labeled
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