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. 2016 May 12;533(7602):251-4.
doi: 10.1038/nature17948. Epub 2016 May 4.

Self-organization of the in vitro attached human embryo

Alessia Deglincerti  1 , Gist F Croft  1 , Lauren N Pietila  1 , Magdalena Zernicka-Goetz  2 , Eric D Siggia  3 , Ali H Brivanlou  1
Affiliations
Free article

Self-organization of the in vitro attached human embryo

Alessia Deglincerti et al. Nature. .
Free article

Abstract

Implantation of the blastocyst is a developmental milestone in mammalian embryonic development. At this time, a coordinated program of lineage diversification, cell-fate specification, and morphogenetic movements establishes the generation of extra-embryonic tissues and the embryo proper, and determines the conditions for successful pregnancy and gastrulation. Despite its basic and clinical importance, this process remains mysterious in humans. Here we report the use of a novel in vitro system to study the post-implantation development of the human embryo. We unveil the self-organizing abilities and autonomy of in vitro attached human embryos. We find human-specific molecular signatures of early cell lineage, timing, and architecture. Embryos display key landmarks of normal development, including epiblast expansion, lineage segregation, bi-laminar disc formation, amniotic and yolk sac cavitation, and trophoblast diversification. Our findings highlight the species-specificity of these developmental events and provide a new understanding of early human embryonic development beyond the blastocyst stage. In addition, our study establishes a new model system relevant to early human pregnancy loss. Finally, our work will also assist in the rational design of differentiation protocols of human embryonic stem cells to specific cell types for disease modelling and cell replacement therapy.

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References

    1. Nat Commun. 2015 Jun 16;6:7329 - PubMed
    1. Dev Cell. 2014 May 27;29(4):454-67 - PubMed
    1. Development. 2013 Oct;140(19):3965-76 - PubMed
    1. Dev Biol. 2013 Mar 1;375(1):54-64 - PubMed
    1. Philos Trans R Soc Lond B Biol Sci. 2003 Aug 29;358(1436):1341-8; discussion 1349 - PubMed

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