Isolation and Identification of Conventional Dendritic Cell Subsets from the Intestine of Mice and Men

doi:10.1007/978-1-4939-3606-9_7

. 2016:1423:101-18.

doi: 10.1007/978-1-4939-3606-9_7.

Isolation and Identification of Conventional Dendritic Cell Subsets from the Intestine of Mice and Men

Charlotte L Scott^{1

2

3}, Pamela B Wright⁴, Simon W F Milling⁵, Allan McI Mowat⁵

Affiliations

¹ Centre for Immunobiology, Institute of Infection, Immunity and Inflammation, College of Veterinary, Medical and Life Sciences, University of Glasgow, Scotland, UK. charlotte.scott@irc.vib-ugent.be.
² Laboratory of Mucosal Immunology and Immunoregulation, Inflammation Research Centre (IRC), VIB Ghent University, Ghent (Zwijnaarde), Belgium. charlotte.scott@irc.vib-ugent.be.
³ Department of Biomedical Molecular Biology, Ghent University Hospital, Ghent, Belgium. charlotte.scott@irc.vib-ugent.be.
⁴ Centre for Immunobiology, Institute of Infection, Immunity and Inflammation, College of Veterinary, Medical and Life Sciences, University of Glasgow, Scotland, UK. pamela.wright@glasgow.ac.uk.
⁵ Centre for Immunobiology, Institute of Infection, Immunity and Inflammation, College of Veterinary, Medical and Life Sciences, University of Glasgow, Scotland, UK.

PMID: 27142011
DOI: 10.1007/978-1-4939-3606-9_7

Isolation and Identification of Conventional Dendritic Cell Subsets from the Intestine of Mice and Men

Charlotte L Scott et al. Methods Mol Biol. 2016.

. 2016:1423:101-18.

doi: 10.1007/978-1-4939-3606-9_7.

Authors

Charlotte L Scott^{1

2

3}, Pamela B Wright⁴, Simon W F Milling⁵, Allan McI Mowat⁵

Affiliations

¹ Centre for Immunobiology, Institute of Infection, Immunity and Inflammation, College of Veterinary, Medical and Life Sciences, University of Glasgow, Scotland, UK. charlotte.scott@irc.vib-ugent.be.
² Laboratory of Mucosal Immunology and Immunoregulation, Inflammation Research Centre (IRC), VIB Ghent University, Ghent (Zwijnaarde), Belgium. charlotte.scott@irc.vib-ugent.be.
³ Department of Biomedical Molecular Biology, Ghent University Hospital, Ghent, Belgium. charlotte.scott@irc.vib-ugent.be.
⁴ Centre for Immunobiology, Institute of Infection, Immunity and Inflammation, College of Veterinary, Medical and Life Sciences, University of Glasgow, Scotland, UK. pamela.wright@glasgow.ac.uk.
⁵ Centre for Immunobiology, Institute of Infection, Immunity and Inflammation, College of Veterinary, Medical and Life Sciences, University of Glasgow, Scotland, UK.

PMID: 27142011
DOI: 10.1007/978-1-4939-3606-9_7

Abstract

The identification of conventional dendritic cells (cDCs) in the intestinal mucosa has been hampered by the difficulties associated with isolating cells from the intestine and by the fact that overlapping markers have made it complicated to discriminate them accurately from other intestinal mononuclear phagocytes such as macrophages (MFs). Here we detail the protocols we have developed to isolate live leukocytes from both murine and human small and large intestines and describe reliable strategies which can be used to identify bona fide cDCs in such preparations.

Keywords: Dendritic cells; Identification; Intestine; Isolation; Subsets.

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Isolation and Identification of Conventional Dendritic Cell Subsets from the Intestine of Mice and Men

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Isolation and Identification of Conventional Dendritic Cell Subsets from the Intestine of Mice and Men

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