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. 2016 May;18(5):491-503.
doi: 10.1038/ncb3333. Epub 2016 Apr 4.

FAK, talin and PIPKIγ regulate endocytosed integrin activation to polarize focal adhesion assembly

Guilherme P F Nader  1 , Ellen J Ezratty  1 , Gregg G Gundersen  1
Affiliations

FAK, talin and PIPKIγ regulate endocytosed integrin activation to polarize focal adhesion assembly

Guilherme P F Nader et al. Nat Cell Biol. 2016 May.

Abstract

Integrin endocytic recycling is critical for cell migration, yet how recycled integrins assemble into new adhesions is unclear. By synchronizing endocytic disassembly of focal adhesions (FAs), we find that recycled integrins reassemble FAs coincident with their return to the cell surface and dependent on Rab5 and Rab11. Unexpectedly, endocytosed integrins remained in an active but unliganded state in endosomes. FAK and Src kinases co-localized with endocytosed integrin and were critical for FA reassembly by regulating integrin activation and recycling, respectively. FAK sustained the active integrin conformation by maintaining talin association with Rab11 endosomes in a type I phosphatidylinositol phosphate kinase (PIPKIγ)-dependent manner. In migrating cells, endocytosed integrins reassembled FAs polarized towards the leading edge, and this polarization required FAK. These studies identify unanticipated roles for FA proteins in maintaining endocytosed integrin in an active conformation. We propose that the conformational memory of endocytosed integrin enhances polarized reassembly of FAs to enable directional cell migration.

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