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. 2016 Feb 20:2016:bav120.
doi: 10.1093/database/bav120. Print 2016.

The UniProtKB guide to the human proteome

Lionel Breuza  1 , Sylvain Poux  2 , Anne Estreicher  2 , Maria Livia Famiglietti  2 , Michele Magrane  3 , Michael Tognolli  2 , Alan Bridge  2 , Delphine Baratin  2 , Nicole Redaschi  2 ; UniProt Consortium
Affiliations

The UniProtKB guide to the human proteome

Lionel Breuza et al. Database (Oxford). .

Abstract

Advances in high-throughput and advanced technologies allow researchers to routinely perform whole genome and proteome analysis. For this purpose, they need high-quality resources providing comprehensive gene and protein sets for their organisms of interest. Using the example of the human proteome, we will describe the content of a complete proteome in the UniProt Knowledgebase (UniProtKB). We will show how manual expert curation of UniProtKB/Swiss-Prot is complemented by expert-driven automatic annotation to build a comprehensive, high-quality and traceable resource. We will also illustrate how the complexity of the human proteome is captured and structured in UniProtKB. Database URL: www.uniprot.org.

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Figures

Figure 1.
Figure 1.
Accessing the human proteome from the UniProt web site (http://www.uniprot.org). A. One can directly type ‘HUMAN’ in the search box. B. Then select ‘Human’ in the ‘Popular organisms’ section on the left.C. There is a single proteome in the ‘Proteomes’ section for this organism, UP000005640, a direct link allowing access to the entries composing the human complete proteome. D. There, one still has the possibility to select the 20 197 expertly ‘Reviewed’ entries of the Swiss-Prot section of UniProtKB from the 49 496 additional ‘unreviewed’ entries of UniProtKB/TrEMBL.
Figure 2.
Figure 2.
Screenshot of the ‘Function’ and ‘Pathology and Biotech’ sections of human FAM20A entry (UniProtKB Q96MK3, http://www.uniprot.org/uniprot/Q96MK3).
Figure 3.
Figure 3.
Annotation scheme for integration of proteomics data in UniProtKB.
See this image and copyright information in PMC

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