OBTECTIVE: To explore the role of transient receptor potential vanilloid subetype 1 (TRPV1) in the increase of the thermal pain threshold by moxibustion.

Methods: Forty Kunming mice (20 ± 2) g were randomized into control group, capsaicin group, capsazepine group, moxibustion group and moxibustion + capsazepine (MC) group with 8 mice in each, and 16 C57BL/6 wild-type mice (18 ± 2) g were randomized into wild-type (WT) control group and WT moxibustion group with 8 mice in each, and 14 TRPV1 knockout mice (18 ± 2) g were randomized into knockout (KO) control group and KO moxibustion-group with 7 in each. Each mouse in the capsaicin group was subcutaneously injected with the amount of 0.1 mL/10 g into L5 and L6 spinal cords; each mouse in the capsazepine group was intraperitoneally injected with the amount of 0.1 mL/10 g. Similarly, each mouse in the moxibustion group was given a suspended moxibustion with specially-made moxa-stick for 20 min on L5 and L6 spinal cords. Each mouse in MC group was intraperitoneally injected with the amount of 0.1 mL/1 0 g first, then after 15 min was given a suspended moxibustion for 20 min on L5 and L6 spinal cords. Each mouse in WT moxibustion group and KO moxibustion group was given a suspended moxibustion with specially-made moxa-stick for 20 min on L5 and L6 spinal cords. The control group, WT control group and KO control group were of no treatment in any way. After all treatments were completed, the digital-display measurement instrument for thermal pain was used to measure the threshold of thermal pain in each group respectively.

Results: Compared with the control group, the thresholds of thermal pain in the moxibustion group and MC group were significantly increased (P <0.01); no significant changes in the thresholds in the capsaicin group and the capsazepine group (P > 0.05); compared with moxibustion group, he threshold of thermal in MC group was obviously decreased (P < 0.01). Compared with WT control group, the threshold of thermal pain in WT moxibustion group was significantly increased (P < 0.01); compared with KO control group, no changes in the threshold in KO moxibustion group (P > 0.05).

Conclusion: TRPV1 participated in the process of increasing the threshold of thermal pain by stimulating L5 and L6 of mice spinal cord with burning mosa-stick.