Next-Generation Sequencing for Diagnosis and Tailored Therapy: A Case Report of Astrovirus-Associated Progressive Encephalitis

doi:10.1093/jpids/piv040

Case Reports

. 2015 Sep;4(3):e53-7.

doi: 10.1093/jpids/piv040. Epub 2015 Jul 12.

Next-Generation Sequencing for Diagnosis and Tailored Therapy: A Case Report of Astrovirus-Associated Progressive Encephalitis

M-L Frémond¹, P Pérot², E Muth³, G Cros¹, M Dumarest², N Mahlaoui⁴, D Seilhean⁵, I Desguerre⁶, C Hébert³, N Corre-Catelin⁷, B Neven¹, M Lecuit⁸, S Blanche¹, C Picard⁹, M Eloit¹⁰

Affiliations

¹ Unité d'Immuno-Hématologie et Rhumatologie Pédiatrique, Hôpital Universitaire Necker-Enfants Malades, Assistance Publique-Hôpitaux de Paris, France Université Paris Descartes, Sorbonne Paris Cité, Institut Imagine, France.
² Institut Pasteur, Biology of Infection Unit, Institut National de la Santé et de la Recherche Médicale (INSERM) U1117, Pathogen Discovery Laboratory, Paris, France.
³ PathoQuest, Paris, France.
⁴ Unité d'Immuno-Hématologie et Rhumatologie Pédiatrique, Hôpital Universitaire Necker-Enfants Malades, Assistance Publique-Hôpitaux de Paris, France Université Paris Descartes, Sorbonne Paris Cité, Institut Imagine, France Laboratoire de Génétique Humaine des Maladies Infectieuses, Branche Necker, INSERM U1163, Paris, France Centre de Référence Déficits Immunitaires Héréditaires, Hôpital Universitaire Necker-Enfants Malades, Assistance Publique-Hôpitaux de Paris, France.
⁵ Département de Neuropathologie, Hôpital Universitaire Pitié-Salpétrière, Université Pierre et Marie Curie, Sorbonne Universités, Paris, France.
⁶ Université Paris Descartes, Unité de Neuropédiatrie, Hôpital Universitaire Necker-Enfants Malades, Paris, France.
⁷ Investigation Clinique et Accès aux Ressources Biologiques, Institut Pasteur, Paris, France.
⁸ Unité d'Immuno-Hématologie et Rhumatologie Pédiatrique, Hôpital Universitaire Necker-Enfants Malades, Assistance Publique-Hôpitaux de Paris, France Paris Descartes University, Sorbonne Paris Cité, Division of Infectious Diseases and Tropical Medicine, Necker-Enfants Malades University Hospital, Institut Imagine, France Institut Pasteur, Biology of Infection Unit, INSERM U1117, Paris, France.
⁹ Unité d'Immuno-Hématologie et Rhumatologie Pédiatrique, Hôpital Universitaire Necker-Enfants Malades, Assistance Publique-Hôpitaux de Paris, France Centre de Référence Déficits Immunitaires Héréditaires, Hôpital Universitaire Necker-Enfants Malades, Assistance Publique-Hôpitaux de Paris, France.
¹⁰ Institut Pasteur, Biology of Infection Unit, Institut National de la Santé et de la Recherche Médicale (INSERM) U1117, Pathogen Discovery Laboratory, Paris, France PathoQuest, Paris, France.

PMID: 26407445
DOI: 10.1093/jpids/piv040

Case Reports

Next-Generation Sequencing for Diagnosis and Tailored Therapy: A Case Report of Astrovirus-Associated Progressive Encephalitis

M-L Frémond et al. J Pediatric Infect Dis Soc. 2015 Sep.

. 2015 Sep;4(3):e53-7.

doi: 10.1093/jpids/piv040. Epub 2015 Jul 12.

Authors

Affiliations

¹ Unité d'Immuno-Hématologie et Rhumatologie Pédiatrique, Hôpital Universitaire Necker-Enfants Malades, Assistance Publique-Hôpitaux de Paris, France Université Paris Descartes, Sorbonne Paris Cité, Institut Imagine, France.
² Institut Pasteur, Biology of Infection Unit, Institut National de la Santé et de la Recherche Médicale (INSERM) U1117, Pathogen Discovery Laboratory, Paris, France.
³ PathoQuest, Paris, France.
⁴ Unité d'Immuno-Hématologie et Rhumatologie Pédiatrique, Hôpital Universitaire Necker-Enfants Malades, Assistance Publique-Hôpitaux de Paris, France Université Paris Descartes, Sorbonne Paris Cité, Institut Imagine, France Laboratoire de Génétique Humaine des Maladies Infectieuses, Branche Necker, INSERM U1163, Paris, France Centre de Référence Déficits Immunitaires Héréditaires, Hôpital Universitaire Necker-Enfants Malades, Assistance Publique-Hôpitaux de Paris, France.
⁵ Département de Neuropathologie, Hôpital Universitaire Pitié-Salpétrière, Université Pierre et Marie Curie, Sorbonne Universités, Paris, France.
⁶ Université Paris Descartes, Unité de Neuropédiatrie, Hôpital Universitaire Necker-Enfants Malades, Paris, France.
⁷ Investigation Clinique et Accès aux Ressources Biologiques, Institut Pasteur, Paris, France.
⁸ Unité d'Immuno-Hématologie et Rhumatologie Pédiatrique, Hôpital Universitaire Necker-Enfants Malades, Assistance Publique-Hôpitaux de Paris, France Paris Descartes University, Sorbonne Paris Cité, Division of Infectious Diseases and Tropical Medicine, Necker-Enfants Malades University Hospital, Institut Imagine, France Institut Pasteur, Biology of Infection Unit, INSERM U1117, Paris, France.
⁹ Unité d'Immuno-Hématologie et Rhumatologie Pédiatrique, Hôpital Universitaire Necker-Enfants Malades, Assistance Publique-Hôpitaux de Paris, France Centre de Référence Déficits Immunitaires Héréditaires, Hôpital Universitaire Necker-Enfants Malades, Assistance Publique-Hôpitaux de Paris, France.
¹⁰ Institut Pasteur, Biology of Infection Unit, Institut National de la Santé et de la Recherche Médicale (INSERM) U1117, Pathogen Discovery Laboratory, Paris, France PathoQuest, Paris, France.

PMID: 26407445
DOI: 10.1093/jpids/piv040

Abstract

A boy with X-linked agammaglobulinemia experienced progressive global motor decline, cerebellar syndrome, and epilepsy. All standard polymerase chain reactions for neurotropic viruses were negative on cerebrospinal fluid and brain biopsy. Next-generation sequencing allowed fast identification of a new astrovirus strain (HAstV-VA1/HMO-C-PA), which led to tailor the patient's treatment, with encouraging clinical monitoring over 1 year.

Keywords: Bruton; agammaglobulinemia; astrovirus; encephalitis.

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Next-Generation Sequencing for Diagnosis and Tailored Therapy: A Case Report of Astrovirus-Associated Progressive Encephalitis

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Next-Generation Sequencing for Diagnosis and Tailored Therapy: A Case Report of Astrovirus-Associated Progressive Encephalitis

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