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. 2015 Oct;10(10):1543-55.
doi: 10.1038/nprot.2015.102. Epub 2015 Sep 17.

MicroRNA-based conversion of human fibroblasts into striatal medium spiny neurons

Michelle Richner  1 , Matheus B Victor  1   2 , Yangjian Liu  1 , Daniel Abernathy  1   3 , Andrew S Yoo  1
Affiliations

MicroRNA-based conversion of human fibroblasts into striatal medium spiny neurons

Michelle Richner et al. Nat Protoc. 2015 Oct.

Abstract

The ability to generate human neurons of specific subtypes of clinical importance offers experimental platforms that may be instrumental for disease modeling. We recently published a study demonstrating the use of neuronal microRNAs (miRNAs) and transcription factors to directly convert human fibroblasts to a highly enriched population of striatal medium spiny neurons (MSNs), a neuronal subpopulation that has a crucial role in motor control and harbors selective susceptibility to cell death in Huntington's disease. Here we describe a stepwise protocol for the generation of MSNs by direct neuronal conversion of human fibroblasts in 30 d. We provide descriptions of cellular behaviors during reprogramming and crucial steps involved in gene delivery, cell adhesion and culturing conditions that promote cell survival. Our protocol offers a unique approach to combine microRNAs and transcription factors to guide the neuronal conversion of human fibroblasts toward a specific neuronal subtype.

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References

    1. J Neurocytol. 1990 Feb;19(1):39-52 - PubMed
    1. Development. 2013 Jan 15;140(2):301-12 - PubMed
    1. PLoS Curr. 2010 Oct 28;2:RRN1193 - PubMed
    1. Mol Cell. 2007 Aug 3;27(3):435-48 - PubMed
    1. Cell. 2011 Jun 10;145(6):827-30 - PubMed

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