Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2015 Oct;17(10):779-87.
doi: 10.1007/s12094-015-1303-1. Epub 2015 Jun 11.

Expression of serum miR-200a, miR-200b, and miR-200c as candidate biomarkers in epithelial ovarian cancer and their association with clinicopathological features

M Zuberi  1 , R Mir  2 , J Das  1 , I Ahmad  1 , J Javid  1 , P Yadav  1 , M Masroor  1 , S Ahmad  1 , P C Ray  1 , A Saxena  3
Affiliations

Expression of serum miR-200a, miR-200b, and miR-200c as candidate biomarkers in epithelial ovarian cancer and their association with clinicopathological features

M Zuberi et al. Clin Transl Oncol. 2015 Oct.

Erratum in

Abstract

Background: MicroRNAs (miRs) have been implicated in the etiology of various human cancers. The aim of this study was to investigate the association of the expression of three members--miR 200a, miR 200b, and miR 200c belonging to the miR-200 family with clinicopathological characteristics and their impact on the progression of epithelial ovarian cancer (EOC).

Materials and methods: Total RNA from serum was isolated by Trizol method, polyadenylated, and reverse transcribed into cDNA. Expression levels of miR-200a, miR-200b, and miR-200c were detected by using miRNA qRT-PCR. We measured miR expression in 70 serum samples of EOC patients with matched controls using U6 snRNA as a reference. Levels of miR expression was compared with distinct clinicopathological features.

Results: Expression of miR-200a was found to be greater than six-fold (p = 0.01), miR-200b and miR-200c greater than three-fold (p = 0.01) in comparison with matched normal controls. Association of miRNA expression with clinicopathological factors and progression was statistically evaluated. The expression levels of miR-200a and miR-200c were found to be significantly associated with disease progression (p = 0.04 and p < 0.001, respectively). miR-200a overexpression was found be associated with tumor histology and stage. Patients with lymph node metastasis showed significant elevation of miR-200c (p = 0.006). The AUC in ROC curve also indicated that serum levels of miR-200a and miR-200c might be worthwhile as a diagnostic tool in the near future.

Conclusion: Our findings suggest that miR-200a, miR-200b, and miR-200c overexpressions are associated with the aggressive tumor progression and be recognized as reliable markers to predict the prognosis and survival in EOC patients.

Keywords: Biomarker; Disease progression; Overexpression; qRT-PCR.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. PLoS One. 2014 Nov 19;9(11):e113700 - PubMed
    1. PLoS One. 2014 Oct 16;9(10):e109508 - PubMed
    1. Int J Mol Sci. 2014 Nov 05;15(11):20134-57 - PubMed
    1. PLoS One. 2009;4(4):e5311 - PubMed
    1. Clin Cancer Res. 2008 May 1;14(9):2690-5 - PubMed

Publication types

MeSH terms

LinkOut - more resources