Role of Capsular Polysaccharides in Biofilm Formation: An AFM Nanomechanics Study
- PMID: 26034816
- DOI: 10.1021/acsami.5b03041
Role of Capsular Polysaccharides in Biofilm Formation: An AFM Nanomechanics Study
Abstract
Bacteria form biofilms to facilitate colonization of biotic and abiotic surfaces, and biofilm formation on indwelling medical devices is a common cause of hospital-acquired infection. Although it is well-recognized that the exopolysaccharide capsule is one of the key bacterial components for biofilm formation, the underlying biophysical mechanism is poorly understood. In the present study, nanomechanical measurements of wild type and specific mutants of the pathogen, Klebsiella pneumoniae, were performed in situ using atomic force microscopy (AFM). Theoretical modeling of the mechanical data and static microtiter plate biofilm assays show that the organization of the capsule can influence bacterial adhesion, and thereby biofilm formation. The capsular organization is affected by the presence of type 3 fimbriae. Understanding the biophysical mechanisms for the impact of the structural organization of the bacterial polysaccharide capsule on biofilm formation will aid the development of strategies to prevent biofilm formation.
Keywords: bacteria; biofilm; capsule; fimbriae; nanomechanics; polysaccharides.
Similar articles
-
Phosphoenolpyruvate phosphotransferase system components positively regulate Klebsiella biofilm formation.J Microbiol Immunol Infect. 2018 Apr;51(2):174-183. doi: 10.1016/j.jmii.2017.01.007. Epub 2017 Jun 29. J Microbiol Immunol Infect. 2018. PMID: 28716362
-
Klebsiella pneumoniae and type 3 fimbriae: nosocomial infection, regulation and biofilm formation.Future Microbiol. 2012 Aug;7(8):991-1002. doi: 10.2217/fmb.12.74. Future Microbiol. 2012. PMID: 22913357 Review.
-
Capsular Polysaccharide Cross-Regulation Modulates Bacteroides thetaiotaomicron Biofilm Formation.mBio. 2020 Jun 23;11(3):e00729-20. doi: 10.1128/mBio.00729-20. mBio. 2020. PMID: 32576670 Free PMC article.
-
Capsule and fimbria interaction in Klebsiella pneumoniae.Infect Immun. 2005 Aug;73(8):4626-33. doi: 10.1128/IAI.73.8.4626-4633.2005. Infect Immun. 2005. PMID: 16040975 Free PMC article.
-
Bacterial Extracellular Polysaccharides in Biofilm Formation and Function.Microbiol Spectr. 2015 Jun;3(3):10.1128/microbiolspec.MB-0011-2014. doi: 10.1128/microbiolspec.MB-0011-2014. Microbiol Spectr. 2015. PMID: 26185074 Free PMC article. Review.
Cited by
-
Pili and other surface proteins influence the structure and the nanomechanical properties of Lactococcus lactis biofilms.Sci Rep. 2021 Mar 1;11(1):4846. doi: 10.1038/s41598-021-84030-1. Sci Rep. 2021. PMID: 33649417 Free PMC article.
-
Co-existence of antibiotic resistance and virulence factors in carbapenem resistant Klebsiella pneumoniae clinical isolates from Alexandria, Egypt.BMC Microbiol. 2024 Nov 11;24(1):466. doi: 10.1186/s12866-024-03600-1. BMC Microbiol. 2024. PMID: 39528926 Free PMC article.
-
Global Dynamic Proteome Study of a Pellicle-forming Acinetobacter baumannii Strain.Mol Cell Proteomics. 2017 Jan;16(1):100-112. doi: 10.1074/mcp.M116.061044. Epub 2016 Oct 31. Mol Cell Proteomics. 2017. PMID: 27799293 Free PMC article.
-
Super-Resolution Fluorescence Microscopy Study of the Production of K1 Capsules by Escherichia coli: Evidence for the Differential Distribution of the Capsule at the Poles and the Equator of the Cell.Langmuir. 2019 Apr 23;35(16):5635-5646. doi: 10.1021/acs.langmuir.8b04122. Epub 2019 Apr 9. Langmuir. 2019. PMID: 30916568 Free PMC article.
-
Investigation of LuxS-mediated quorum sensing in Klebsiella pneumoniae.J Med Microbiol. 2020 Mar;69(3):402-413. doi: 10.1099/jmm.0.001148. J Med Microbiol. 2020. PMID: 32223838 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Miscellaneous
