Transcription factor E2F is required for efficient expression of the hamster dihydrofolate reductase gene in vitro and in vivo
- PMID: 2601705
- PMCID: PMC363651
- DOI: 10.1128/mcb.9.11.4994-5002.1989
Transcription factor E2F is required for efficient expression of the hamster dihydrofolate reductase gene in vitro and in vivo
Abstract
The dihydrofolate reductase (DHFR) gene encodes an enzyme important for metabolism and cell growth. We have found multiple DNA-protein interactions within the hamster DHFR gene promoter in vitro. These interactions occur over the consensus binding sites for two eucaryotic transcription factors. Sp1 and E2F. The DHFR E2F consensus site possesses a dyad symmetry and is unique in its location immediately 3' to the major transcription start site. The interaction of E2F with the DHFR promoter has been detected in HeLa nuclear extracts, confirmed by using partially purified E2F, and characterized by both enzymatic and chemical assays of the DNA-protein interaction. A mutation of the E2F recognition sequence which abolishes E2F binding to the DHFR promoter results in a two- to fivefold decrease of in vitro transcriptional activity and a fivefold reduction of DHFR promoter activity in transient-expression assays. Thus, the interaction of E2F with the DHFR promoter is required for efficient expression of the DHFR gene.
Similar articles
-
Protein-DNA interactions at the major and minor promoters of the divergently transcribed dhfr and rep3 genes during the Chinese hamster ovary cell cycle.Mol Cell Biol. 1996 Feb;16(2):634-47. doi: 10.1128/MCB.16.2.634. Mol Cell Biol. 1996. PMID: 8552092 Free PMC article.
-
Position-dependent transcriptional regulation of the murine dihydrofolate reductase promoter by the E2F transactivation domain.Mol Cell Biol. 1997 Apr;17(4):1966-76. doi: 10.1128/MCB.17.4.1966. Mol Cell Biol. 1997. PMID: 9121444 Free PMC article.
-
Cell-growth regulation of the hamster dihydrofolate reductase gene promoter by transcription factor Sp1.Eur J Biochem. 1997 Oct 1;249(1):13-20. doi: 10.1111/j.1432-1033.1997.00013.x. Eur J Biochem. 1997. PMID: 9363748
-
Transcriptional regulation of the dihydrofolate reductase/rep-3 locus.Crit Rev Eukaryot Gene Expr. 1994;4(1):19-53. doi: 10.1615/critreveukargeneexpr.v4.i1.20. Crit Rev Eukaryot Gene Expr. 1994. PMID: 7987046 Review.
-
Transcription from TATA-less promoters: dihydrofolate reductase as a model.Crit Rev Eukaryot Gene Expr. 1993;3(4):229-54. Crit Rev Eukaryot Gene Expr. 1993. PMID: 8286846 Review.
Cited by
-
Genetic analysis of the adenovirus E4 6/7 trans activator: interaction with E2F and induction of a stable DNA-protein complex are critical for activity.J Virol. 1991 Oct;65(10):5364-73. doi: 10.1128/JVI.65.10.5364-5373.1991. J Virol. 1991. PMID: 1832724 Free PMC article.
-
Cell cycle analysis of E2F in primary human T cells reveals novel E2F complexes and biochemically distinct forms of free E2F.Mol Cell Biol. 1993 Jul;13(7):3975-83. doi: 10.1128/mcb.13.7.3975-3983.1993. Mol Cell Biol. 1993. PMID: 8321204 Free PMC article.
-
The adenovirus E1A-associated kinase consists of cyclin E-p33cdk2 and cyclin A-p33cdk2.J Virol. 1993 May;67(5):2456-65. doi: 10.1128/JVI.67.5.2456-2465.1993. J Virol. 1993. PMID: 8386263 Free PMC article.
-
Deregulated expression of E2F-1 induces S-phase entry and leads to apoptosis.Mol Cell Biol. 1994 Dec;14(12):8166-73. doi: 10.1128/mcb.14.12.8166-8173.1994. Mol Cell Biol. 1994. PMID: 7969153 Free PMC article.
-
E1A 12S and 13S of the transformation-defective adenovirus type 12 strain CS-1 inactivate proteins of the RB family, permitting transactivation of the E2F-dependent promoter.J Virol. 1997 Dec;71(12):9538-48. doi: 10.1128/JVI.71.12.9538-9548.1997. J Virol. 1997. PMID: 9371617 Free PMC article.
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
