Regnase-1 and Roquin Regulate a Common Element in Inflammatory mRNAs by Spatiotemporally Distinct Mechanisms

doi:10.1016/j.cell.2015.04.029

. 2015 May 21;161(5):1058-1073.

doi: 10.1016/j.cell.2015.04.029.

Regnase-1 and Roquin Regulate a Common Element in Inflammatory mRNAs by Spatiotemporally Distinct Mechanisms

Takashi Mino¹, Yasuhiro Murakawa², Akira Fukao³, Alexis Vandenbon⁴, Hans-Hermann Wessels², Daisuke Ori¹, Takuya Uehata⁵, Sarang Tartey¹, Shizuo Akira⁶, Yutaka Suzuki⁷, Carola G Vinuesa⁸, Uwe Ohler², Daron M Standley⁹, Markus Landthaler², Toshinobu Fujiwara³, Osamu Takeuchi¹⁰

Affiliations

¹ Laboratory of Infection and Prevention, Institute for Virus Research, Kyoto University, Kyoto 606-8507, Japan; CREST, JST, Kyoto 606-8507, Japan.
² Max Delbrück Center for Molecular Medicine, Berlin Institute for Medical Systems Biology, 13125 Berlin, Germany.
³ Laboratory of Hygienic Chemistry, Graduate School of Pharmaceutical Sciences, Nagoya City University, Nagoya 467-8603, Japan.
⁴ Laboratory of Systems Immunology, Osaka University, Suita, Osaka 565-0871, Japan; Immuno-genomics Research Unit, Osaka University, Suita, Osaka 565-0871, Japan.
⁵ Laboratory of Infection and Prevention, Institute for Virus Research, Kyoto University, Kyoto 606-8507, Japan.
⁶ Laboratory of Host Defense, WPI Immunology Frontier Research Center, Osaka University, Suita, Osaka 565-0871, Japan; Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565-0871, Japan.
⁷ Laboratory of Functional Genomics, Department of Medical Genome Sciences, Graduate School of Frontier Sciences, The University of Tokyo, Chiba 277-8562, Japan.
⁸ Department of Pathogens and Immunity, John Curtin School of Medical Research, Australian National University, Canberra, ACT 0200, Australia.
⁹ Laboratory of Systems Immunology, Osaka University, Suita, Osaka 565-0871, Japan; Laboratory of Integrated Biological Information, Institute for Virus Research, Kyoto University, Kyoto 606-8507, Japan.
¹⁰ Laboratory of Infection and Prevention, Institute for Virus Research, Kyoto University, Kyoto 606-8507, Japan; CREST, JST, Kyoto 606-8507, Japan. Electronic address: otake@virus.kyoto-u.ac.jp.

PMID: 26000482
DOI: 10.1016/j.cell.2015.04.029

Free article

Regnase-1 and Roquin Regulate a Common Element in Inflammatory mRNAs by Spatiotemporally Distinct Mechanisms

Takashi Mino et al. Cell. 2015.

Free article

. 2015 May 21;161(5):1058-1073.

doi: 10.1016/j.cell.2015.04.029.

Authors

Affiliations

¹ Laboratory of Infection and Prevention, Institute for Virus Research, Kyoto University, Kyoto 606-8507, Japan; CREST, JST, Kyoto 606-8507, Japan.
² Max Delbrück Center for Molecular Medicine, Berlin Institute for Medical Systems Biology, 13125 Berlin, Germany.
³ Laboratory of Hygienic Chemistry, Graduate School of Pharmaceutical Sciences, Nagoya City University, Nagoya 467-8603, Japan.
⁴ Laboratory of Systems Immunology, Osaka University, Suita, Osaka 565-0871, Japan; Immuno-genomics Research Unit, Osaka University, Suita, Osaka 565-0871, Japan.
⁵ Laboratory of Infection and Prevention, Institute for Virus Research, Kyoto University, Kyoto 606-8507, Japan.
⁶ Laboratory of Host Defense, WPI Immunology Frontier Research Center, Osaka University, Suita, Osaka 565-0871, Japan; Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565-0871, Japan.
⁷ Laboratory of Functional Genomics, Department of Medical Genome Sciences, Graduate School of Frontier Sciences, The University of Tokyo, Chiba 277-8562, Japan.
⁸ Department of Pathogens and Immunity, John Curtin School of Medical Research, Australian National University, Canberra, ACT 0200, Australia.
⁹ Laboratory of Systems Immunology, Osaka University, Suita, Osaka 565-0871, Japan; Laboratory of Integrated Biological Information, Institute for Virus Research, Kyoto University, Kyoto 606-8507, Japan.
¹⁰ Laboratory of Infection and Prevention, Institute for Virus Research, Kyoto University, Kyoto 606-8507, Japan; CREST, JST, Kyoto 606-8507, Japan. Electronic address: otake@virus.kyoto-u.ac.jp.

PMID: 26000482
DOI: 10.1016/j.cell.2015.04.029

Abstract

Regnase-1 and Roquin are RNA binding proteins essential for degradation of inflammation-related mRNAs and maintenance of immune homeostasis. However, their mechanistic relationship has yet to be clarified. Here, we show that, although Regnase-1 and Roquin regulate an overlapping set of mRNAs via a common stem-loop structure, they function in distinct subcellular locations: ribosome/endoplasmic reticulum and processing-body/stress granules, respectively. Moreover, Regnase-1 specifically cleaves and degrades translationally active mRNAs and requires the helicase activity of UPF1, similar to the decay mechanisms of nonsense mRNAs. In contrast, Roquin controls translationally inactive mRNAs, independent of UPF1. Defects in both Regnase-1 and Roquin lead to large increases in their target mRNAs, although Regnase-1 tends to control the early phase of inflammation when mRNAs are more actively translated. Our findings reveal that differential regulation of mRNAs by Regnase-1 and Roquin depends on their translation status and enables elaborate control of inflammation.

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Regnase-1 and Roquin Regulate a Common Element in Inflammatory mRNAs by Spatiotemporally Distinct Mechanisms

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Regnase-1 and Roquin Regulate a Common Element in Inflammatory mRNAs by Spatiotemporally Distinct Mechanisms

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