A restricted cytoplasmic region of IL-2 receptor beta chain is essential for growth signal transduction but not for ligand binding and internalization
- PMID: 2590941
- DOI: 10.1016/0092-8674(89)90607-7
A restricted cytoplasmic region of IL-2 receptor beta chain is essential for growth signal transduction but not for ligand binding and internalization
Abstract
The functional, high affinity form of interleukin-2 receptor (IL-2R) is composed of two receptor components, the IL-2R alpha (p55) and IL-2R beta (p70-75) chains. Unlike the IL-2R alpha chain, the IL-2R beta chain contains a large cytoplasmic domain that shows no obvious tyrosine kinase motif. In the present study, we report the establishment of a system in which the cDNA-directed human IL-2R beta allows growth signal transduction in a mouse pro-B cell line. This system enabled us to identify a unique region within the cytoplasmic domain of the human IL-2R beta chain essential for ligand-mediated signal transduction. We also demonstrate that certain cytoplasmic deletion mutants in the IL-2R beta chain, although deficient in signal transduction, can still form high affinity IL-2R in conjunction with endogenous mouse IL-2R alpha chain; the mutants are still able to internalize the ligand as well.
Similar articles
-
Characterization of the heterodimeric complex of human IL-2 receptor alpha.beta chains reconstituted in a mouse fibroblast cell line, L929.J Immunol. 1990 Oct 1;145(7):2177-82. J Immunol. 1990. PMID: 2398275
-
Structure and function of IL-2 and IL-2 receptors.Behring Inst Mitt. 1992 Apr;(91):87-95. Behring Inst Mitt. 1992. PMID: 1524574 Review.
-
Ligand binding by the IL-2 receptor is modulated by intracellular determinants of the IL-2 receptor beta-chain.J Immunol. 1995 Mar 1;154(5):2033-40. J Immunol. 1995. PMID: 7868880
-
Growth signal transduction by the human interleukin-2 receptor requires cytoplasmic tyrosines of the beta chain and non-tyrosine residues of the gamma c chain.J Biol Chem. 1995 Sep 15;270(37):21729-37. doi: 10.1074/jbc.270.37.21729. J Biol Chem. 1995. PMID: 7665592
-
The human interleukin-2 receptor: insights into subunit structure and growth signal transduction.Semin Immunol. 1990 Mar;2(2):119-28. Semin Immunol. 1990. PMID: 2129904 Review.
Cited by
-
Functional dissection of p56lck, a protein tyrosine kinase which mediates interleukin-2-induced activation of the c-fos gene.Mol Cell Biol. 1994 Sep;14(9):5812-9. doi: 10.1128/mcb.14.9.5812-5819.1994. Mol Cell Biol. 1994. PMID: 8065316 Free PMC article.
-
Distinct cytoplasmic regions of the human granulocyte colony-stimulating factor receptor involved in induction of proliferation and maturation.Mol Cell Biol. 1993 Dec;13(12):7774-81. doi: 10.1128/mcb.13.12.7774-7781.1993. Mol Cell Biol. 1993. PMID: 8246993 Free PMC article.
-
c-fos gene induction by interleukin 2: identification of the critical cytoplasmic regions within the interleukin 2 receptor beta chain.Proc Natl Acad Sci U S A. 1992 Mar 15;89(6):2022-6. doi: 10.1073/pnas.89.6.2022. Proc Natl Acad Sci U S A. 1992. PMID: 1549560 Free PMC article.
-
Murine interleukin 2 receptor beta chain: dysregulated gene expression in lymphoma line EL-4 caused by a promoter insertion.Proc Natl Acad Sci U S A. 1990 Mar;87(5):1806-10. doi: 10.1073/pnas.87.5.1806. Proc Natl Acad Sci U S A. 1990. PMID: 2155425 Free PMC article.
-
Analysis of the biochemical mechanisms for the endocrine actions of fibroblast growth factor-23.Endocrinology. 2005 Nov;146(11):4647-56. doi: 10.1210/en.2005-0670. Epub 2005 Aug 4. Endocrinology. 2005. PMID: 16081635 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
