Comparative Study
doi: 10.1016/0014-5793(85)80753-5.
Affinity of protein HU for different nucleic acids
- PMID: 2581810
- DOI: 10.1016/0014-5793(85)80753-5
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Comparative Study
Affinity of protein HU for different nucleic acids
FEBS Lett.
.
Free article
Abstract
The binding of protein HU from Escherichia coli to nucleic acids was investigated by affinity chromatography under various conditions, by a nitrocellulose retention assay and by isopycnic centrifugations in metrizamide gradients. The results indicate that HU has a preference for binding to RNA and single-stranded DNA over double-stranded DNA. The affinity of HU for supercoiled DNA was also less than that of the corresponding relaxed DNA.
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