doi: 10.1126/science.2579430.
Neoplastic transformation of human epidermal keratinocytes by AD12-SV40 and Kirsten sarcoma viruses
- PMID: 2579430
- DOI: 10.1126/science.2579430
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Neoplastic transformation of human epidermal keratinocytes by AD12-SV40 and Kirsten sarcoma viruses
Science.
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Abstract
Recent investigations have begun to dissect the number and nature of genetic alterations associated with cancer cells. In the present study, primary human epidermal keratinocytes acquired indefinite life-span in culture but did not undergo malignant conversion in response to infection with a hybrid of adenovirus 12 and simian virus 40. Addition of Kirsten murine sarcoma virus, which contains a K-ras oncogene, to these cells induced morphological alterations associated with the acquisition of neoplastic properties. These findings demonstrate the malignant transformation of human primary epithelial cells in culture and support a multiple-step process for neoplastic conversion.
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