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. 1989 Feb;25(2):193-6.
doi: 10.1007/BF02626177.

Maintaining mosquito cell lines at high temperatures: effects on the replication of flaviviruses

G Kuno  1 , A Oliver
Affiliations

Maintaining mosquito cell lines at high temperatures: effects on the replication of flaviviruses

G Kuno et al. In Vitro Cell Dev Biol. 1989 Feb.

Abstract

The upper thermal limit for maintenance of eleven mosquito cell lines was studied. Although most cell lines could be grown at 32 degrees C to 34 degrees C, Anopheles stephensi cell line could be maintained at 37 degrees C. At higher temperatures initial growth rate was higher, but yield of cells after about a week of incubation was lower than at the standard temperature (28 degrees C). Replication of several flaviviruses in Aedes albopictus cell cultures adapted to 34.5 degrees C was faster, and viral titers were higher than at 28 degrees C.

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