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. 2015 Jan:62:1-5.
doi: 10.1016/j.jcv.2014.11.011. Epub 2014 Nov 15.

The emergence of enterovirus D68 in a Dutch University Medical Center and the necessity for routinely screening for respiratory viruses

Randy Poelman  1 , Elisabeth H Schölvinck  2 , Renze Borger  1 , Hubert G M Niesters  1 , Coretta van Leer-Buter  3
Affiliations

The emergence of enterovirus D68 in a Dutch University Medical Center and the necessity for routinely screening for respiratory viruses

Randy Poelman et al. J Clin Virol. 2015 Jan.

Abstract

Background: Since August 2014, an increase in infections caused by enterovirus D68 (EV-D68) was reported in the USA and Canada, for the most part in children presenting with severe respiratory symptoms.

Objectives: To determine whether an increase in severe EV-D68 respiratory infections was observed in our region.

Study design: Samples from patients with respiratory symptoms were screened for viral pathogens, including rhinovirus and enterovirus. Subsequently, samples positive for rhinovirus and enterovirus were routinely sequenced for phylogenetic analysis. Furthermore, an additional method was used to detect EV-D68 specifically.

Results: During the first three quarters of the year 2014, 1896 respiratory samples were analyzed; 39 (2%) of them tested positive for enterovirus. Eighteen samples tested positive for EV-D68, obtained from 16 different patients admitted to our hospital. Eleven were children below the age of 18, of whom five children needed intensive care treatment. The remaining five samples were from adults, who all had an underlying disease; three were transplant patients (heart, lung and renal transplantation), the other two had an underlying lung condition (COPD, asthma). Phylogenetic analysis showed a close relationship with the strains circulating currently in the USA, all belonging to the known EV-D68 genetic subtypes.

Conclusions: We observed an increase of EV-D68 infections in our population, both in children as well as in adult. In 2014 there have been 16 cases so far, compared to none in 2011 and 2013 and a single case in 2012. Phylogenetic analysis identified two similar clusters as shown in the USA and Canada.

Keywords: Enterovirus D68; Respiratory infections; Routinely screening; VP1 sequencing.

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Figures

Fig. 1
Fig. 1
EV-D68 occurrence in the UMCG 2009–2014.
Fig. 2
Fig. 2
Phylogenetic tree EV-D68 2009–2014. Node colors – purple: 2009, red: 2010, yellow: 2011, light blue: 2012, green: 2014. Clades A, B and C as defined by Tokarz et al. .
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References

    1. Centers for Disease Control and Prevention . 2014. cdc.gov. http://www.cdc.gov/non-polio-enterovirus/outbreaks/EV-D68-outbreaks.html [accessed 24.10.14]
    1. Rahamat-Langendoen J., Riezebos-Brilman A., Borger R., van der Heide R., Brandenburg A., Schölvinck E. Upsurge of human enterovirus 68 infections in patients with severe respiratory tract infections. J Clin Virol. 2011;52:103–106. - PubMed
    1. Niesters H.G.M., Rossen J.W., van der Avoort H., Baas D., Benschop K., Claas E.C. Laboratory-based surveillance in the molecular era: the TYPENED model, a joint data-sharing platform for clinical and public health laboratories. Euro Surveill. 2013;18(4) pii 20387. - PubMed
    1. European Centre for Disease Prevention and Control . ECDC; Stockholm: 2014. Enterovirus D68 detections in the USA and Canada – first update 15 October 2014.
    1. Meijer A., Benschop K.S., Donker G.A., van der Avoort H.G. Continued seasonal circulation of enterovirus D68 in the Netherlands, 2011–2014. Euro Surveill. 2014;19(42) pii 20935. - PubMed

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