Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2015 Mar;9(2):59-63.
doi: 10.1111/irv.12300. Epub 2014 Dec 23.

High frequency of enterovirus D68 in children hospitalised with respiratory illness in Norway, autumn 2014

Karoline Bragstad  1 , Kirsti Jakobsen, Astrid E Rojahn, Marius K Skram, Kirsti Vainio, Mona Holberg-Petersen, Olav Hungnes, Susanne G Dudman, Anne-Marte B Kran
Affiliations

High frequency of enterovirus D68 in children hospitalised with respiratory illness in Norway, autumn 2014

Karoline Bragstad et al. Influenza Other Respir Viruses. 2015 Mar.

Abstract

Objectives: An unexpectedly high proportion of children were admitted for severe respiratory infections at the Oslo University Hospital, Ullevål, Norway, during September and October, 2014. In light of the ongoing outbreak of enterovirus-D68 (EV-D68) in North America a real-time RT-PCR for screening of enterovirus and enterovirus D68 was established.

Design: We developed a duplex real-time RT-PCR for rapid screening of enterovirus D68. The method target the 5' non-translated region (NTR) of the HEV genome at a location generally used for enterovirus detection.

Sample: Nasopharyngeal samples (n = 354), from children <15 years of age, received for respiratory virus analysis in OUH during September 1st and October 31nd, 2014, were tested for enterovirus and screened for enterovirus D68.

Main outcome measures and results: The duplex real-time RT-PCR method was an efficient tool for rapid screening for EV-D68 in respiratory specimens. Enterovirus was detected in 66 (22%) of 303 pediatric nasopharyngeal samples collected from children hospitalised with acute respiratory infection within the two-month period. Out of these, 33 (50%) were EV-D68. EV-D68 was associated with acute flaccid paralysis in one child.

Conclusions: An unexpectedly high proportion of children admitted for severe respiratory infections at the Oslo University Hospital, Ullevål, Norway, were diagnosed with EV- D68 during September 1st and October 31nd, 2014. These results emphasise that greater vigilance is required throughout Europe as enteroviruses are cause of severe respiratory disease.

Keywords: Acute flaccid paralysis; enterovirus D68; picornavirus; respiratory infections; respiratory viruses.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Fluorescents diagram (A) showing detection sensitivity of EV-D68 and standard curve (B) with 10-fold dilution series of EV-D68 RNA in duplicates (undiluted to 10−8) detected by the EV-D68 probe in the duplex EV-EVD68 real-time RT-PCR assay.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Schieble JH, Fox VL, Lennette EH. A probable new human picornavirus associated with respiratory diseases. Am J Epidemiol. 1967;85:297–310. - PubMed
    1. Midgley CM, Jackson MA, Selvarangan R, et al. Severe respiratory illness associated with enterovirus D68 – Missouri and Illinois, 2014. MMWR Morb Mortal Wkly Rep. 2014;63:798–799. - PMC - PubMed
    1. Meijer A, Benschop KS, Donker GA, van der Avoort HG. Continued seasonal circulation of enterovirus D68 in the Netherlands, 2011-2014. Euro Surveill. 2014;19:20935. - PubMed
    1. Solomon T, Willison H. Infectious causes of acute flaccid paralysis. Curr Opin Infect Dis. 2003;16:375–381. - PubMed
    1. Lang M, Mirand A, Savy N, et al. Acute flaccid paralysis following enterovirus D68 associated pneumonia, France, 2014. Euro Surveill. 2014;19:20952. - PubMed

MeSH terms

LinkOut - more resources