Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 1989 Jul;42(7):749-54.
doi: 10.1136/jcp.42.7.749.

Detection of cytomegalovirus antigens and DNA in tissues fixed in formaldehyde

N M Jiwa  1 , A K Raap, F M van de Rijke, A Mulder, J J Weening, F E Zwaan, T H The, M van der Ploeg
Affiliations
Comparative Study

Detection of cytomegalovirus antigens and DNA in tissues fixed in formaldehyde

N M Jiwa et al. J Clin Pathol. 1989 Jul.

Abstract

Immunohistochemical techniques with monoclonal antibodies against cytomegalovirus (CMV) immediate early (IEA) and early antigens (EA), and in situ hybridisation, were used to detect CMV infection in routinely obtained, formaldehyde fixed and paraffin wax embedded tissues taken from bone marrow transplant patients, who had died form interstitial pneumonia. To improve the rates of detection of CMV-IEA and EA the wax embedded material was pretreated with 0.4% pepsin/HCl at 37 degrees C for 30 minutes. This pretreatment was also advantageous for in situ hybridisation. In the patients with histological evidence of CMV infection or positive viral culture from the lung tissue, or both, viral proteins and nucleic acids were detected in lung, as well as in other organs. Immunohistochemical techniques proved superior in heavily infected but necrotic tissues. In control patients (patients who had died from interstitial pneumonia without any evidence of CMV, or with no interstitial pneumonia at all) in situ hybridisation showed no positive signal, while immunohistochemical techniques showed only a few positive cells in lung tissue of one of nine patients. In addition to CMV-DNA analysis, formaldehyde-fixed, paraffin wax embedded tissue is amenable to immunohistochemical analysis with CMV monoclonal antibodies.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. J Immunol Methods. 1977;15(2):163-7 - PubMed
    1. J Med Virol. 1988 Jun;25(2):179-88 - PubMed
    1. Am J Clin Pathol. 1982 Jan;77(1):90-4 - PubMed
    1. Transplantation. 1982 Mar;33(3):265-8 - PubMed
    1. Hum Pathol. 1982 May;13(5):411-5 - PubMed

Publication types

LinkOut - more resources