Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1989 Aug;63(8):3330-7.
doi: 10.1128/JVI.63.8.3330-3337.1989.

Identification and expression of a human cytomegalovirus early glycoprotein

C P Chang  1 , D H Vesole, J Nelson, M B Oldstone, M F Stinski
Affiliations

Identification and expression of a human cytomegalovirus early glycoprotein

C P Chang et al. J Virol. 1989 Aug.

Abstract

A human cytomegalovirus early gene which possesses three temporally regulated promoters is located in the large unique component of the viral genome between 0.054 and 0.064 map units (C.-P. Chang, C.L. Malone, and M.F. Stinski, J. Virol. 63:281-290, 1989). This gene contains a major open reading frame (ORF) located 233 bases downstream of the cap site of an early unspliced RNA. The major ORF predicts a polypeptide of 17 kilodaltons (kDa) which contains a glycoproteinlike signal and anchor domains as well as potential N-glycosylation sites. Antisera were prepared against synthetic peptides derived from amino acid sequences within the major ORF. The antisera detected a viral glycoprotein of 48 kDa in infected cells and recognized the in vitro-translated 17-kDa protein early-gene product. The viral glycoprotein, designated gp48, was modified by N-linked glycans and possibly O-linked glycans. The synthesis of gp48 occurred in the absence of viral DNA replication but accumulated to the highest levels at late times after infection. Since gp48 was found in the virion, it is considered an early structural glycoprotein.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Methods Enzymol. 1980;65(1):499-560 - PubMed
    1. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 - PubMed
    1. J Virol. 1982 Feb;41(2):462-77 - PubMed
    1. J Virol. 1984 Jan;49(1):132-41 - PubMed
    1. Proc Natl Acad Sci U S A. 1984 Feb;81(3):876-80 - PubMed

Publication types

MeSH terms

Associated data

LinkOut - more resources