doi: 10.1016/0166-0934(89)90129-8.
A rapid microneutralization assay for the measurement of neutralizing antibody reactive with human cytomegalovirus
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- PMID: 2542350
- DOI: 10.1016/0166-0934(89)90129-8
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A rapid microneutralization assay for the measurement of neutralizing antibody reactive with human cytomegalovirus
J Virol Methods.
1989 Feb.
Abstract
We have developed a murine monoclonal antibody reactive with a major immediate early 72,000 dalton protein of human cytomegalovirus and utilized this reagent in a rapid virus titration and microneutralization assay. Because of the early expression of this virus encoded protein, both assays could be accomplished within 16 h following virus inoculation. In addition, both assays resulted in considerable savings of reagents because the assays were carried out in 96-well microtiter plates. These assays should prove useful in the preparation and study of neutralizing antibodies directed against human cytomegalovirus.
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