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. 1989 May;86(9):3384-8.
doi: 10.1073/pnas.86.9.3384.

Role of oligosaccharides in the processing and maturation of envelope glycoproteins of human immunodeficiency virus type 1

R Pal  1 , G M Hoke, M G Sarngadharan
Affiliations

Role of oligosaccharides in the processing and maturation of envelope glycoproteins of human immunodeficiency virus type 1

R Pal et al. Proc Natl Acad Sci U S A. 1989 May.

Abstract

The processing and maturation of envelope glycoproteins of human immunodeficiency virus type 1 (HIV-1) were studied in infected cells treated with inhibitors of oligosaccharide processing. In MOLT-3 cells chronically infected with HIV-1 (strain HTLV-IIIB), tunicamycin severely inhibited the glycosylation of envelope proteins. Deoxynojirimycin, an inhibitor of glucosidase I in the rough endoplasmic reticulum, inhibited the proteolytic processing of gp160, whereas no such effect was noted with either deoxymannojirimycin or swainsonine, inhibitors of mannosidase I and II, respectively, in the Golgi complex. The processed gp120 and gp41 synthesized in the presence of deoxymannojirimycin were found to contain mannose-rich oligosaccharide cores as evidenced by their susceptibility to endoglycosidase H digestion. The formation of syncytia normally observed when CEM cells are cocultured with HIV-1-infected cells was markedly inhibited in the presence of deoxynojirimycin, but such inhibition was not observed in cells treated with deoxymannojirimycin or swainsonine. The infectivity of virions released from MOLT-3/HTLV-IIIB cells treated with deoxynojirimycin or deoxymannojirimycin was significantly lower than the infectivity of virions released from untreated cells. On the other hand, treatment with swainsonine did not affect the infectivity of the progeny virus. These results suggest that the proteolytic processing of gp160 takes place in infected cells when the glycoprotein has mannose-rich oligosaccharide structures. Trimming of glucose residues and the primary trimming of mannose residues are necessary for the release of infectious virus.

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References

    1. AIDS Res Hum Retroviruses. 1987 Fall;3(3):265-82 - PubMed
    1. Proc Natl Acad Sci U S A. 1987 Nov;84(22):8120-4 - PubMed
    1. J Biol Chem. 1988 Aug 25;263(24):11760-7 - PubMed
    1. J Virol. 1988 Sep;62(9):3167-74 - PubMed
    1. Proc Natl Acad Sci U S A. 1988 Dec;85(23):9248-52 - PubMed

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