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. 2014 Sep 15;7(10):6486-92.
eCollection 2014.

DSTYK kinase domain ablation impaired the mice capabilities of learning and memory in water maze test

Kui Li  1 , Ji-Wei Liu  2 , Zhi-Chuan Zhu  2 , Hong-Tao Wang  2 , Yong Zu  2 , Yong-Jie Liu  2 , Yan-Hong Yang  2 , Zhi-Qi Xiong  2 , Xu Shen  3 , Rui Chen  4 , Jing Zheng  2 , Ze-Lan Hu  2
Affiliations

DSTYK kinase domain ablation impaired the mice capabilities of learning and memory in water maze test

Kui Li et al. Int J Clin Exp Pathol. .

Abstract

DSTYK (Dual serine/threonine and tyrosine protein kinase) is a putative dual Ser/Thr and Tyr protein kinase with unique structural features. It is proposed that DSTYK may play important roles in brain because of its high expression in most brain areas. In the present study, a DSTYK knockout (KO) mouse line with the ablation of C-terminal of DSTYK including the kinase domain was generated to study the physiological function of DSTYK. The DSTYK KO mice are fertile and have no significant morphological defects revealed by Nissl staining compared with wildtype mice. Open field test and rotarod test showed there is no obvious difference in basic motor and balance capacity between the DSTYK homozygous KO mice and DSTYK heterozygous KO mice. In water maze test, however, the DSTYK homozygous KO mice show impaired capabilities of learning and memory compared with the DSTYK heterozygous KO mice.

Keywords: DSTYK; brain development; knockout mice; learning and memory.

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Figures

Figure 1
Figure 1
Schematic diagram showing the knockout strategy of DSTYK in mice. Exons 4-13 was removed from mouse genomic DNA leading to the kinase domain ablation of DSTYK protein. The sites for binding of genotyping Primers (P1-P4) were also showed.
Figure 2
Figure 2
Genotyping, mRNA expression and protein expression of mice with kinase domain ablation of DSTYK. A. Representative Genotyping PCR results using different primer sets. B. The mRNA level of DSTYK in DSTYK+/+, DSTYK+/- and DSTYK-/- mice analyzed by quantitative PCR. Sample size was marked in the columns. C. DSTYK protein expression in DSTYK+/+ and DSTYK-/- mice assessed by western blot.
Figure 3
Figure 3
Representative Nissl staining of brain sections showed no significant morphological defects of mouse brain.
Figure 4
Figure 4
DSTYK knockout mice showed no significant defects in basic motor and balance capacity. A. Total distance moved in open field test. B. Rota rod test showed no significant difference between DSTYK heterozygous and homozygous knockout mice. N.S. No Significance.
Figure 5
Figure 5
Visible task results of water maze test. A. DSTYK-/- mice showed a longer Latency to target platform than DSTYK+/- mice in training round 3 and 4. B. There is no significant difference in average speed between DSTYK+/- mice and DSTYK-/- mice. “*”, P < 0.05.
Figure 6
Figure 6
Probe trial results of water maze test. A. Target platform latency. B. Number of target platform crossing. C. Time spent in target quadrant. D. Relative total distance moved. DSTYK+/-, n=9; DSTYK-/-, n=14. “***”, P < 0.001.
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