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. 2014 Oct 7:3:204.
doi: 10.4103/2277-9175.142314. eCollection 2014.

Taurine improves the wound healing process in cutaneous leishmaniasis in mice model, based on stereological parameters

Soheil Ashkani-Esfahani  1 , Fariba Zarifi  1 , Qasem Asgari  2 , Asal Zargari Samadnejad  1 , Shima Rafiee  1 , Ali Noorafshan  3
Affiliations

Taurine improves the wound healing process in cutaneous leishmaniasis in mice model, based on stereological parameters

Soheil Ashkani-Esfahani et al. Adv Biomed Res. .

Abstract

Background: Cutaneous Leishmaniasis is a self-limiting disease caused by protozoan parasites of the genus Leishmania, which affects the skin with full-thickness wounds, which are prone to scar formation even after treatment. Taurine (Tu) is one of the most abundant amino acids that has antioxidant and anti-inflammatory effects, which play an important role in the process of wound healing. Herein, we have investigated the effects of Tu on cutaneous Leishmaniasis wounds and L. major promastigotes.

Materials and methods: Eighteen mice were induced with Leishmaniasis wounds (with L. Major) on the base of their tails and divided into three groups, T1: Treated with Tu injection, T2: Treated with Tu gel, and C: No treatment. Treatments were carried out every 24 hours for 21 days. The volume densities of the collagen bundles and vessels, vessel's length density and diameter, and fibroblast populations were estimated by stereological methods. Flow cytometry was used in order to investigate the direct Tu effect on parasites. The Mann-Whitney U test was used and P ≤ 0.05 was considered to be statistically significant.

Results: The numerical density of the fibroblasts, volume density of the collagen bundles, and length densities of the vessels in groups T1 and T2 were significantly higher than in group C (P < 0.05). The fibroblast numerical density of group T1 was higher than that of group T2 (P = 0.02). Incidentally, Tu had no direct effect on L. major parasites according to the flow cytometry analysis.

Conclusion: Tu showed the ability to improve the wound healing process and tissue regeneration although it had no direct anti-leishmaniasis effect.

Keywords: Cutaneous leishmaniasis; mice; stereology; taurine; wound healing.

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Conflict of interest statement

Conflict of Interest: None declared.

Figures

Figure 1
Figure 1
The effect of Taurine (Tu) on the wound closure rate in control, topical Tu-treated, and injected Tu-treated mice, with cutaneous leishmaniasis. Each point represents the mean ± SD of the six wounds. The wound closure rate had significantly increased in both the Tu-treated groups compared to the control group (P < 0.05). The ‘a’ letters show a significant difference compared to the control group on each day
Figure 2
Figure 2
Effect of different concentrations of Tu and 70% alcohol (as a control) on parasite mortality. (Horizontal apex: Concentration, vertical apex: Mortality)
See this image and copyright information in PMC

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