The effect of human C-reactive protein on the cell-attachment activity of fibronectin and laminin
- PMID: 2521604
- DOI: 10.1016/0014-4827(89)90059-1
The effect of human C-reactive protein on the cell-attachment activity of fibronectin and laminin
Abstract
We have previously reported that purified human C-reactive protein (CRP) specifically binds to the cell-binding region of plasma fibronectin (Fn) in a Ca2+-dependent reaction that is saturable at a molar ratio of CRP/Fn of approximately 9. In this study, the binding of CRP to Fn was found to interfere with the cell-attachment promoting activity of Fn. The inhibition of cell attachment was dependent on the concentration of the CRP and involved the phosphorylcholine (PC) binding site of CRP since inhibition was prevented by allowing the CRP to react with either PC (or closely related monophosphate compounds) or a mAb specific for the PC-binding site of CRP. Binding of CRP to laminin was also Ca2+-dependent; however, this binding did not alter the cell-attachment promoting activity of laminin. CRP by itself does not mediate cell attachment. Since CRP is selectively deposited at sites of tissue damage along with plasma Fn and has the ability to bind to Fn and alter its cell-binding activity, CRP may modulate early events in tissue repair.
Similar articles
-
Binding of human C-reactive protein (CRP) to plasma fibronectin occurs via the phosphorylcholine-binding site.Mol Immunol. 1988 Aug;25(8):679-86. doi: 10.1016/0161-5890(88)90103-4. Mol Immunol. 1988. PMID: 2460754
-
Characteristics of the binding of human C-reactive protein (CRP) to laminin.J Cell Biochem. 1989 May;40(1):121-32. doi: 10.1002/jcb.240400112. J Cell Biochem. 1989. PMID: 2745572
-
A cell attachment peptide from human C-reactive protein.J Cell Biochem. 1992 Sep;50(1):83-92. doi: 10.1002/jcb.240500113. J Cell Biochem. 1992. PMID: 1429876
-
Recent advances in research on fibronectin and other cell attachment proteins.J Cell Biochem. 1985;28(2):79-97. doi: 10.1002/jcb.240280202. J Cell Biochem. 1985. PMID: 3001108 Review. No abstract available.
-
Laminin: multiple forms, multiple receptors.Curr Opin Cell Biol. 1990 Oct;2(5):845-9. doi: 10.1016/0955-0674(90)90082-p. Curr Opin Cell Biol. 1990. PMID: 2150589 Review. No abstract available.
Cited by
-
Molecular genetics, structure, and function of C-reactive protein.Immunol Res. 1991;10(1):43-53. doi: 10.1007/BF02918166. Immunol Res. 1991. PMID: 1865130 Review. No abstract available.
-
Variations in binding characteristics of glycosylated human C-reactive proteins in different pathological conditions.Glycoconj J. 2004;20(9):537-43. doi: 10.1023/B:GLYC.0000043290.90182.e6. Glycoconj J. 2004. PMID: 15454692
-
A hypothesis resolving the apparently disparate activities of native and altered forms of human C-reactive protein.Immunol Res. 1993;12(1):37-47. doi: 10.1007/BF02918367. Immunol Res. 1993. PMID: 8515183 Review.
-
Identification of acidic pH-dependent ligands of pentameric C-reactive protein.J Biol Chem. 2010 Nov 12;285(46):36235-44. doi: 10.1074/jbc.M110.142026. Epub 2010 Sep 14. J Biol Chem. 2010. PMID: 20843812 Free PMC article.
-
Humoral innate immunity at the crossroad between microbe and matrix recognition: The role of PTX3 in tissue damage.Semin Cell Dev Biol. 2017 Jan;61:31-40. doi: 10.1016/j.semcdb.2016.07.026. Epub 2016 Jul 29. Semin Cell Dev Biol. 2017. PMID: 27476448 Free PMC article. Review.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Research Materials
Miscellaneous