Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2014 Dec;89(12):E235-8.
doi: 10.1002/ajh.23837. Epub 2014 Oct 25.

Resveratrol increases the bone marrow hematopoietic stem and progenitor cell capacity

Pauline Rimmelé  1 , Sébastien Lofek-Czubek, Saghi Ghaffari
Affiliations

Resveratrol increases the bone marrow hematopoietic stem and progenitor cell capacity

Pauline Rimmelé et al. Am J Hematol. 2014 Dec.

Abstract

Resveratrol is a plant-derived polyphenol that has shown protective effects against many disorders including, several types of cancers and other age-associated diseases as well as blood disorders in cultured cells and/or animal models. However, whether resveratrol has any impact specifically on normal blood stem cells remains unknown. Here, we show that a 3-week treatment of resveratrol increases the frequency and total numbers of normal bone marrow hematopoietic stem cells (HSC) without any impact on their competitive repopulation capacity. In addition, we show that resveratrol enhances the bone marrow multipotent progenitor capacity in vivo. These results have therapeutic value for disorders of hematopoietic stem and progenitor cells (HSPC) as well as for bone marrow transplantation settings.

PubMed Disclaimer

Conflict of interest statement

The authors do not have any conflict of interest to declare.

Figures

Figure 1
Figure 1. Resveratrol treatment increases HSPC numbers in the BM
A. HSC phenotype and function are analyzed in wild type mice treated with vehicle control (Ct) or 5mg/kg/day resveratrol (Res) for three weeks (left panel). Total number of BM cells per femur (n=12 mice) (right panel). B. Lineage negative (Lin−) BM cells were analyzed for c-Kit versus Sca-1 expression (LSK). Representative flow cytometry plots of LSK frequency from A are shown. (C) Frequencies (left panel) and numbers (right panel) per femur of LSK cells (n=12 mice). D. Representative flow cytometry plots of LSKCD48−CD150+ (LT-HSC) frequency from A are shown. E. Frequencies (left panel) and numbers (right panel) per femur of LT-HSC (n=6 mice).
Figure 2
Figure 2. Resveratrol treatment does not modulate significantly the competitive repopulation ability of LT-HSC
LT-HSC (100 CD45.2 cells) isolated as in Figure 1D were mixed with 2.105 total BM cells (CD45.1) and transplanted into lethally irradiated recipients (CD45.1, n=8), and the contribution to the peripheral blood formation of (CD45.2) cells was evaluated at shown time points. All data are expressed as mean ± SEM (*p<0.05).
Figure 3
Figure 3. Resveratrol increases multipotential myeloid progenitor cell compartment
A. Mice were treated for three weeks with vehicle control (Ct) or 5mg/kg/day resveratrol (Res). BM cells were isolated and assayed for CFU-S in lethally irradiated mice. CFU-S-derived colonies were measured 12 days after injection of 105 WT BM into lethally irradiated hosts (n=4 mice in each group). One representative of two independent experiments is shown. All data are expressed as mean±SEM (*p<0.05). B. Isolated BM cells (1,5 ×105) (n=6 mice) were cultured in semi-solid methylcellulose in duplicates and the number of colony-forming cell-derived colonies (BFUE CFU-GM and CFU-GEMM) was measured.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Raederstorff D, Kunz I, Schwager J. Resveratrol, from experimental data to nutritional evidence: the emergence of a new food ingredient. Ann N Y Acad Sci. 2013;1290:136–141. - PubMed
    1. Wood JG, Rogina B, Lavu S, et al. Sirtuin activators mimic caloric restriction and delay ageing in metazoans. Nature. 2004;430:686–689. - PubMed
    1. Howitz KT, Bitterman KJ, Cohen HY, et al. Small molecule activators of sirtuins extend Saccharomyces cerevisiae lifespan. Nature. 2003;425:191–196. - PubMed
    1. Jang M, Cai L, Udeani GO, et al. Cancer chemopreventive activity of resveratrol, a natural product derived from grapes. Science. 1997;275:218–220. - PubMed
    1. Baur JA, Pearson KJ, Price NL, et al. Resveratrol improves health and survival of mice on a high-calorie diet. Nature. 2006;444:337–342. - PMC - PubMed

Publication types

MeSH terms