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. 2014 Jul;20(7):1263-4.
doi: 10.3201/eid2007.140500.

Stability of Middle East respiratory syndrome coronavirus in milk

Neeltje van Doremalen, Trenton Bushmaker, William B Karesh, Vincent J Munster

Stability of Middle East respiratory syndrome coronavirus in milk

Neeltje van Doremalen et al. Emerg Infect Dis. 2014 Jul.
No abstract available

Keywords: MERS-CoV; Middle East respiratory syndrome coronavirus; foodborne transmission; milk; stability; viruses; zoonoses.

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Figures

Figure
Figure
Viability of MERS-CoV in unpasteurized milk. MERS-CoV strain Jordan-N3/2012 was diluted in milk or DMEM to a final TCID50 of 105.5/mL and stored at either 4°C (A) or 22°C (B). MERS-CoV titer was determined at 0, 8, 24, 48, and 72 hours post dilution in quintuplicate. Red indicates dromedary camel milk; blue indicates goat milk; green indicates cow milk; black indicates DMEM. C) Milk containing MERS-CoV was pasteurized by heating 1-mL aliquots of diluted virus at 63°C for 30 min in triplicate. Red indicates unpasteurized; blue indicates pasteurized. Infectious virus titers were determined by endpoint titration on Vero E6 cells in triplicate. Dotted line depicts the detection limit of the assay. MERS-CoV, Middle East respiratory syndrome coronavirus; TCID50, 50% tissue culture infective dose; DMEM, Dulbecco modified Eagle medium.
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