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. 2014 Jun;7(6):1772-1776.
doi: 10.3892/etm.2014.1652. Epub 2014 Mar 31.

Combined application of alginate dressing and human granulocyte-macrophage colony stimulating factor promotes healing in refractory chronic skin ulcers

Guobao Huang  1 , Tangqing Sun  2 , Lei Zhang  2 , Qiuhe Wu  2 , Keyan Zhang  2 , Qingfen Tian  2 , Ran Huo  3
Affiliations

Combined application of alginate dressing and human granulocyte-macrophage colony stimulating factor promotes healing in refractory chronic skin ulcers

Guobao Huang et al. Exp Ther Med. 2014 Jun.

Abstract

The aim of the present study was to evaluate the clinical therapeutic effect of the combined application of alginate and recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF) on the healing of refractory chronic skin ulcers. A single center, three arm, randomized study was performed at Jinan Central Hospital (Jinan, Shandong, China). A total of 60 patients with refractory chronic skin ulcers, which persisted for >1 month, were enrolled and randomly assigned into one of the following three groups: alginate dressing/rhGM-CSF group (group A), rhGM-CSF only group (group B) and conventional (vaseline dressing) group (group C). The wound area rate was measured, granulation and color were observed and pain was evaluated. The data were summarized and statistical analysis was performed. The results demonstrated that group A exhibited a significantly faster wound healing rate and lower pain score compared with the other groups (P<0.01). In conclusion, the combined application of alginate dressing and rhGM-CSF for the treatment of refractory chronic skin ulcers demonstrated significant advantages. It promoted the growth of granulation tissue, accelerated re-epithelialization and also effectively reduced wound pain, and thus improved the quality of life for the patient. This suggests that the combined application of alginate and rhGM-CSF may be an effective therapeutic strategy for the clinical treatment of refractory chronic skin ulcers.

Keywords: GM-CSF; alginate; diabetic foot; refractory skin ulcer.

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Figures

Figure 1
Figure 1
Quantified evaluation of wound healing using the GHS based on visual observations of patients in groups A (solid line), B (dashed line) and C (dashed dot line). GHS was calculated as the average score of a group at an evaluation time point. Statistical analysis demonstrated significant differences among the three groups (P<0.001). GHS, group healing score.
Figure 2
Figure 2
Representative images of pressure sores from patients in the three treatment groups at different time points. A, B and C indicate the treatment group that each image represents and the number indicates the number of days of treatment. The images demonstrate wound surface color, granulation and excretion, however, the wound surface area is not demonstrated since the magnitude of each image may be different.
Figure 4
Figure 4
Pain evaluation was performed using the VAS method. Patients in groups A (solid line), B (dashed line) and C (dashed dot line) were asked to assess their pain on a scale each time the dressing was changed. The data were plotted at different time points. Statistical analysis demonstrated significant differences among the three groups (P<0.001). VAS, visual analogue scale.
Figure 3
Figure 3
Wound healing rate, calculated by measuring the wound area at designated time points, is shown for patients in groups A (solid line), B (dashed line) and C (dashed dot line). The wound area was measured each time the dressing was changed. The length of the longest diameter and the vertical diameter were obtained. Statistical analysis demonstrated significant differences among the three groups (P<0.001).
See this image and copyright information in PMC

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References

    1. Barnard J, Millner R. A review of topical hemostatic agents for use in cardiac surgery. Ann Thorac Surg. 2009;88:1377–1383. - PubMed
    1. Niemi T, Svartling N, Syrjälä M, Asko-Seljavaara S, Rosenberg P. Haemostatic disturbances in burned patients during early excision and skin grafting. Blood Coagul Fibrinolysis. 1998;9:19–28. - PubMed
    1. Wee S, Gombotz WR. Protein release from alginate matrices. Adv Drug Deliv Rev. 1998;31:267–285. - PubMed
    1. Groenewold MD, Gribnau AJ, Ubbink DT. Topical haemostatic agents for skin wounds: a systematic review. BMC Surg. 2011;11:15. - PMC - PubMed
    1. Rabbany SY, Pastore J, Yamamoto M, Miller T, Rafii S, Aras R, Penn M. Continuous delivery of stromal cell-derived factor-1 from alginate scaffolds accelerates wound healing. Cell Transplant. 2010;19:399–408. - PubMed

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