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Review
. 2014 Aug:7:66-72.
doi: 10.1016/j.coviro.2014.04.004. Epub 2014 May 10.

Viral miRNA targeting of bicistronic and polycistronic transcripts

Ying Zhu  1 , Yufei Huang  2 , Jae U Jung  1 , Chun Lu  3 , Shou-Jiang Gao  4
Affiliations
Review

Viral miRNA targeting of bicistronic and polycistronic transcripts

Ying Zhu et al. Curr Opin Virol. 2014 Aug.

Abstract

Successful viral infection entails a choreographic regulation of viral gene expression program. Kaposi's sarcoma-associated herpesvirus (KSHV) encodes numerous miRNAs that regulate viral life cycle. However, few viral targets have been identified due to the lack of information on KSHV 3' untranslated regions (3'UTRs). Recent genome-wide mapping of KSHV transcripts and 3'UTRs has revealed abundant bicistronic and polycistronic transcripts. The extended 3'UTRs of the 5' proximal genes of bicistronic and polycistronic transcripts offer additional regulatory targets. Indeed, a genome-wide screening of KSHV 3'UTRs has identified several bicistronic and polycistronic transcripts as the novel targets of viral miRNAs. Together, these works have expanded our knowledge of the unique features of KSHV gene regulation program and provided valuable resources for the research community.

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Figures

Figure 1
Figure 1
KSHV 3′UTRs and targets of KSHV miRNAs. Genes are labeled in blue with arrowheads indicating the transcription orientation. Intergenic regions are labeled in gray. 3′UTRs of viral transcripts are indicated as purple lines. Transcripts targeted by KSHV miRNAs are marked with the respective miRNAs in red with bicistronic and polycistronic transcripts marked with “*”. miR-K3-5p targeting sites in the ORF30-33 cluster are marked with yellow rectangles while miR-K10a/b-3p targeting sites in the ORF71-73 cluster are marked with red rectangles. Adapted from [73••].
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