The miR-29b-Sirt1 axis regulates self-renewal of mouse embryonic stem cells in response to reactive oxygen species

doi:10.1016/j.cellsig.2014.03.010

. 2014 Jul;26(7):1500-5.

doi: 10.1016/j.cellsig.2014.03.010. Epub 2014 Mar 18.

The miR-29b-Sirt1 axis regulates self-renewal of mouse embryonic stem cells in response to reactive oxygen species

Zengguang Xu¹, Lei Zhang², Xuejie Fei³, Xiuwen Yi⁴, Wenxian Li⁵, Qingxiu Wang⁶

Affiliations

¹ Research Center for Translational Medicine, East Hospital, Tongji University School of Medicine, Shanghai 200120, People's Republic of China; Department of Preventive Medicine, Tongji University School of Medicine, East Hospital, Shanghai 200120, People's Republic of China.
² Research Center for Translational Medicine, East Hospital, Tongji University School of Medicine, Shanghai 200120, People's Republic of China; Department of Anesthesiology, East Hospital, Tongji University School of Medicine, 150 Jimo Road, Shanghai 200120, People's Republic of China.
³ Department of Hospital Infections, Shanghai Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, People's Republic of China.
⁴ Department of Anesthesiology, The Eye, Ear, Nose, and Throat Hospital, Fudan University, Shanghai 200031, People's Republic of China.
⁵ Department of Anesthesiology, The Eye, Ear, Nose, and Throat Hospital, Fudan University, Shanghai 200031, People's Republic of China. Electronic address: wenxianli66@gmail.com.
⁶ Department of Anesthesiology, East Hospital, Tongji University School of Medicine, 150 Jimo Road, Shanghai 200120, People's Republic of China. Electronic address: xuzg1998@126.com.

PMID: 24657470
DOI: 10.1016/j.cellsig.2014.03.010

The miR-29b-Sirt1 axis regulates self-renewal of mouse embryonic stem cells in response to reactive oxygen species

Zengguang Xu et al. Cell Signal. 2014 Jul.

. 2014 Jul;26(7):1500-5.

doi: 10.1016/j.cellsig.2014.03.010. Epub 2014 Mar 18.

Authors

Zengguang Xu¹, Lei Zhang², Xuejie Fei³, Xiuwen Yi⁴, Wenxian Li⁵, Qingxiu Wang⁶

Affiliations

¹ Research Center for Translational Medicine, East Hospital, Tongji University School of Medicine, Shanghai 200120, People's Republic of China; Department of Preventive Medicine, Tongji University School of Medicine, East Hospital, Shanghai 200120, People's Republic of China.
² Research Center for Translational Medicine, East Hospital, Tongji University School of Medicine, Shanghai 200120, People's Republic of China; Department of Anesthesiology, East Hospital, Tongji University School of Medicine, 150 Jimo Road, Shanghai 200120, People's Republic of China.
³ Department of Hospital Infections, Shanghai Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, People's Republic of China.
⁴ Department of Anesthesiology, The Eye, Ear, Nose, and Throat Hospital, Fudan University, Shanghai 200031, People's Republic of China.
⁵ Department of Anesthesiology, The Eye, Ear, Nose, and Throat Hospital, Fudan University, Shanghai 200031, People's Republic of China. Electronic address: wenxianli66@gmail.com.
⁶ Department of Anesthesiology, East Hospital, Tongji University School of Medicine, 150 Jimo Road, Shanghai 200120, People's Republic of China. Electronic address: xuzg1998@126.com.

PMID: 24657470
DOI: 10.1016/j.cellsig.2014.03.010

Abstract

Endogenous reactive oxygen species (ROS) control is important for the maintenance of self-renewal of embryonic stem (ES) cells. Although miRNAs have been found to be critically involved in the regulation of the self-renewal, whether miRNAs can regulate the signaling axis to control ROS in ES cells is unclear. Here we show that miR-29b specifically regulates the self-renewal of mouse ES cells in response to ROS generated by antioxidant-free culture. Sirt1 is the direct target of miR-29b and can also make mES cells sensitive to ROS and regulate the self-renewal of mES cells during the response of ROS. We further found that Sirt1 could attenuate the miR-29b function in regulating mES cells' self-renewal in response to ROS. Our results determined that miR-29b-Sirt1 axis regulates self-renewal of mES cells in response to ROS.

Keywords: Mouse embryonic stem cell; Reactive oxygen species; Self-renewal; Sirt1; miR-29b.

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The miR-29b-Sirt1 axis regulates self-renewal of mouse embryonic stem cells in response to reactive oxygen species

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The miR-29b-Sirt1 axis regulates self-renewal of mouse embryonic stem cells in response to reactive oxygen species

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