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. 2014 Mar 3;9(3):e88922.
doi: 10.1371/journal.pone.0088922. eCollection 2014.

Timing of intermittent seminal HIV-1 RNA shedding in patients with undetectable plasma viral load under combination antiretroviral therapy

Xavier Ferraretto  1 , Candice Estellat  2 , Florence Damond  3 , Pascale Longuet  4 , Sylvie Epelboin  5 , Pauline Demailly  6 , Chadi Yazbeck  5 , Marie-Astrid Llabador  6 , Blandine Pasquet  2 , Yazdan Yazdanpanah  7 , Sophie Matheron  7 , Catherine Patrat  6
Affiliations

Timing of intermittent seminal HIV-1 RNA shedding in patients with undetectable plasma viral load under combination antiretroviral therapy

Xavier Ferraretto et al. PLoS One. .

Abstract

It was demonstrated that combination antiretroviral therapy (cART) reduces the HIV-1 viral load (VL) in the blood and the seminal compartment. Some studies have reported that the seminal HIV-1 VL is undetectable in individuals with an undetectable blood plasma viral load (bpVL) under cART. However, some recent studies have demonstrated that seminal HIV-1 RNA may still be detected, and potentially infectious, even in the case of an undetectable bpVL. The aim of this retrospective study was to determine the detection rate of a seminal VL and whether shedding could be intermittent over a very short time. From January 2006 to December 2011, 88 HIV-1 infected men, enrolled in an Assisted Reproduction program, provided 306 semen samples, corresponding to 177 frozen sperm samples (two samples delivered at a one-hour interval (n = 129) or one sample (n = 48)). All enrolled men were under cART, with an undetectable bpVL for more than 6 months. HIV-1 RNA was quantified in seminal plasma using a Roche COBAS Ampliprep COBAS TaqMan HIV-1 test. Seminal HIV-1 RNA was detected in 23 samples (7.5%) from 17 patients (19.3%). This detection rate was stable over years. With regards to the freezing of two samples delivered at a one-hour interval, the proportion of discordance between the first and second samples was 9.3% (12/129). Our results confirm the intermittent shedding of HIV-1 in semen. While this finding has been shown by studies examining longer time intervals, to our knowledge, this has never been demonstrated over such a short time interval.

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Conflict of interest statement

Competing Interests: The authors have declared that no competing interests exist.

Figures

Figure 1
Figure 1. Pattern of HIV-1 shedding in the semen of 17 patients with at least one detectable seminal plasma viral load.
Each horizontal line represents the data for one subject. Seminal plasma viral loads are represented by circles coloured as follows: white, undetectable; dark grey, detectable. The viral loads (copies/ml) of detectable samples are annotated near the dark grey circles. Two juxtaposed circles represent samples provided at a one-hour interval.
Figure 2
Figure 2. Prevalence of detectable seminal plasma samples (dark grey) and of men with at least one detectable spVL (light grey) from 2006 to 2011.
Bars represent the 95% confidence intervals.
See this image and copyright information in PMC

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References

    1. Cazein F, Le Strat Y, Le Vu S, Pillonel J, Lot F, et al. (2012) HIV testing in France, 2003–2011. BEH 46–47: 529–533.
    1. Boily M, Baggaley R, Wang L, Masse B, White R, et al. (2009) Heterosexual risk of HIV-1 infection per sexual act: systematic review and meta-analysis of observational studies. Lancet Infect Dis 9: 118–129. - PMC - PubMed
    1. Semprini A, Levi-Setti P, Bozzo M, Ravizza M, Taglioretti A, et al. (1992) Insemination of HIV-negative women with processed semen of HIV-positive partners. Lancet 340: 1317–1319. - PubMed
    1. Bujan L, Hollander L, Coudert M, Gilling-Smith C, Vucetich A, et al. (2007) Safety and efficacy of sperm washing in HIV-1-serodiscordant couples where the male is infected: results from the European CREAThE network. AIDS 21: 1909–1914. - PubMed
    1. Leruez-Ville M, Dulioust E, Costagliola D, Salmon D, Tachet A, et al. (2002) Decrease in HIV-1 seminal shedding in men receiving highly active antiretroviral therapy: an 18 month longitudinal study (ANRS EP012). AIDS 16: 486–488. - PubMed

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