Nucleic acid sequence relationships between enterovirus serotypes
- PMID: 2456458
- DOI: 10.1016/0890-8508(87)90025-9
Nucleic acid sequence relationships between enterovirus serotypes
Abstract
Forty-eight different enterovirus serotypes were analysed by a nucleic-acid hybridization test using probes derived from the 3' end of coxsackievirus A21 (CA21) and B3 (CB3), poliovirus 3 (P3) and enterovirus 70 (E70). More than 90% of the serotypes could be detected with this collection of reagents. The CB3 probe reacted with all the coxsackie B viruses, with all three poliovirus serotypes, and with almost all of the 30 ECHO virus types tested. In addition some of the coxsackie A viruses and the BrDr 73 strain of enterovirus 71 gave a positive signal with this probe. The P3 probe detected all the poliovirus strains and also some coxsackievirus A isolates but no coxsackie B or ECHO viruses. A similar hybridization pattern as with the P3 probe was obtained when the CA 21 probe was used. The E70 probe appeared to be strain-specific. The results indicate that nucleic-acid hybridization is a useful method for rapid detection and subgrouping of enteroviruses during virus isolation, and that the test could be further developed for typing of the strains.
Similar articles
-
Genetic diversity of enterovirus subgroups.Arch Virol. 1989;104(3-4):175-86. doi: 10.1007/BF01315541. Arch Virol. 1989. PMID: 2539795
-
Detection of enteroviruses using subgenomic probes of Coxsackie virus B4 by hybridization.Diagn Microbiol Infect Dis. 1988 Nov;11(3):129-36. doi: 10.1016/0732-8893(88)90014-4. Diagn Microbiol Infect Dis. 1988. PMID: 2854510
-
Detection of enteroviruses in faeces by polymerase chain reaction.Scand J Infect Dis. 1992;24(3):265-73. doi: 10.3109/00365549209061331. Scand J Infect Dis. 1992. PMID: 1324520
-
Enterovirus infections with special reference to enterovirus 71.J Microbiol Immunol Infect. 2000 Mar;33(1):1-8. J Microbiol Immunol Infect. 2000. PMID: 10806956 Review.
-
Role of recombination in evolution of enteroviruses.Rev Med Virol. 2005 May-Jun;15(3):157-67. doi: 10.1002/rmv.457. Rev Med Virol. 2005. PMID: 15578739 Review.
Cited by
-
Molecular detection and identification of an enterovirus during an outbreak of aseptic meningitis.J Clin Lab Anal. 2001;15(2):87-95. doi: 10.1002/jcla.7. J Clin Lab Anal. 2001. PMID: 11291111 Free PMC article.
-
Parechoviruses.J Virol. 1999 Jul;73(7):5249-54. doi: 10.1128/JVI.73.7.5249-5254.1999. J Virol. 1999. PMID: 10364270 Free PMC article. Review. No abstract available.
-
Use of monoclonal antibodies to identify serotypes of enterovirus isolates.J Clin Microbiol. 1998 Jul;36(7):1877-81. doi: 10.1128/JCM.36.7.1877-1881.1998. J Clin Microbiol. 1998. PMID: 9650928 Free PMC article.
-
Detection of enteroviruses in groundwater with the polymerase chain reaction.Appl Environ Microbiol. 1993 May;59(5):1318-24. doi: 10.1128/aem.59.5.1318-1324.1993. Appl Environ Microbiol. 1993. PMID: 7685998 Free PMC article.
-
Detection of enteroviruses using cDNA and synthetic oligonucleotide probes.J Virol Methods. 1989 Aug;25(2):233-40. doi: 10.1016/0166-0934(89)90035-9. J Virol Methods. 1989. PMID: 2550504 Free PMC article.