Cloning and transcriptional analysis of a variant surface glycoprotein gene expression site in Trypanosoma brucei
- PMID: 2455225
- DOI: 10.1016/0166-6851(88)90004-7
Cloning and transcriptional analysis of a variant surface glycoprotein gene expression site in Trypanosoma brucei
Abstract
The variant surface glycoprotein (VSG) gene expression site in Trypanosoma brucei variant 117a has been mapped to a point about 40 kb upstream from the VSG gene. Sequences upstream from the previously identified [Cully, D.F. et al. (1985) Cell 42, 173-182] expression site associated gene (ESAG-I) have been cloned and a stable 1.3 kb transcript has been localized immediately 5' to ESAG-I. This transcript is in the same orientation and approximately as abundant as the ESAG-I message. A highly conserved region, of which at least 15 copies are present in the genome, has been identified further upstream. A stable transcript corresponding to this region was not detected in variant 117a, but a 1.7 kb transcript was detected in variant 221a. In isolated nuclei, representative sequences from the 117a, expression site were transcribed unidirectionally at similar rates, and transcription was insensitive to alpha-amanitin.
Similar articles
-
Expression site-associated genes transcribed independently of variant surface glycoprotein genes in Trypanosoma brucei.Mol Biochem Parasitol. 1991 Jul;47(1):31-41. doi: 10.1016/0166-6851(91)90145-v. Mol Biochem Parasitol. 1991. PMID: 1713298
-
Coordinate transcription of variant surface glycoprotein genes and an expression site associated gene family in Trypanosoma brucei.Cell. 1985 Aug;42(1):173-82. doi: 10.1016/s0092-8674(85)80113-6. Cell. 1985. PMID: 2861910
-
The anatomy and transcription of a monocistronic expression site for a metacyclic variant surface glycoprotein gene in Trypanosoma brucei.J Biol Chem. 1999 Jun 11;274(24):16876-83. doi: 10.1074/jbc.274.24.16876. J Biol Chem. 1999. PMID: 10358033
-
Regulation of antigen gene expression in Trypanosoma brucei.Trends Parasitol. 2005 Nov;21(11):517-20. doi: 10.1016/j.pt.2005.08.016. Epub 2005 Aug 29. Trends Parasitol. 2005. PMID: 16126458 Review.
-
Nuclear gene transcription and chromatin in Trypanosoma brucei.Int J Parasitol. 2001 Sep;31(11):1157-65. doi: 10.1016/s0020-7519(01)00264-8. Int J Parasitol. 2001. PMID: 11513885 Review.
Cited by
-
The promoter for a variant surface glycoprotein gene expression site in Trypanosoma brucei.EMBO J. 1990 Sep;9(9):2791-801. doi: 10.1002/j.1460-2075.1990.tb07467.x. EMBO J. 1990. PMID: 1697265 Free PMC article.
-
Characterization of the RNA polymerases of Trypanosoma brucei: trypanosomal mRNAs are composed of transcripts derived from both RNA polymerase II and III.EMBO J. 1989 Nov;8(11):3383-9. doi: 10.1002/j.1460-2075.1989.tb08502.x. EMBO J. 1989. PMID: 2583103 Free PMC article.
-
The genes encoding fructose bisphosphate aldolase in Trypanosoma brucei are interspersed with unrelated genes.Nucleic Acids Res. 1990 May 25;18(10):2967-75. doi: 10.1093/nar/18.10.2967. Nucleic Acids Res. 1990. PMID: 2349093 Free PMC article.
-
A transferrin-binding protein of Trypanosoma brucei is encoded by one of the genes in the variant surface glycoprotein gene expression site.EMBO J. 1991 May;10(5):1061-6. doi: 10.1002/j.1460-2075.1991.tb08045.x. EMBO J. 1991. PMID: 1845367 Free PMC article.
-
A family of genes related to a new expression site-associated gene in Trypanosoma equiperdum.Mol Cell Biol. 1991 Apr;11(4):2180-8. doi: 10.1128/mcb.11.4.2180-2188.1991. Mol Cell Biol. 1991. PMID: 1672441 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
