Synthetic amphiphilic peptide models for protein ion channels
- PMID: 2453923
- DOI: 10.1126/science.2453923
Synthetic amphiphilic peptide models for protein ion channels
Erratum in
- Science 1989 Sep 29;245(4925):1437
Abstract
Ion channel proteins are important for the conduction of ions across biological membranes. Recent analyses of their sequences have suggested that they are composed of bundles of alpha-helices that associate to form ion-conducting channels. To gain insight into the mechanisms by which alpha-helices can aggregate and conduct ions, three model peptides containing only leucine and serine residues were synthesized and characterized. A 21-residue peptide, H2N-(Leu-Ser-Ser-Leu-Leu-Ser-Leu)3-CONH2, which was designed to be a membrane-spanning amphiphilic alpha-helix, formed well-defined ion channels with ion permeability and lifetime characteristics resembling the acetylcholine receptor. In contrast, a 14-residue version of this peptide, which was too short to span the phospolipid bilayer as an alpha-helix, failed to form discrete, stable channels. A third peptide, H2N-(Leu-Ser-Leu-Leu-Leu-Ser-Leu)3-CONH2, in which one serine per heptad repeat was replaced by leucine, produced proton-selective channels. Computer graphics and energy minimization were used to create molecular models that were consistent with the observed properties of the channels.
Similar articles
-
Conformationally constrained alpha-helical peptide models for protein ion channels.Biopolymers. 1990 Jan;29(1):205-13. doi: 10.1002/bip.360290125. Biopolymers. 1990. PMID: 1691664
-
Topological equilibria of ion channel peptides in oriented lipid bilayers revealed by 15N solid-state NMR spectroscopy.Biochemistry. 2005 Sep 13;44(36):12120-7. doi: 10.1021/bi050866n. Biochemistry. 2005. PMID: 16142910
-
A helical-dipole model describes the single-channel current rectification of an uncharged peptide ion channel.Proc Natl Acad Sci U S A. 1994 May 24;91(11):4859-63. doi: 10.1073/pnas.91.11.4859. Proc Natl Acad Sci U S A. 1994. PMID: 7515180 Free PMC article.
-
Peptide ion channels: design and creation of function.Biopolymers. 1998;47(1):75-81. doi: 10.1002/(SICI)1097-0282(1998)47:1<75::AID-BIP8>3.0.CO;2-U. Biopolymers. 1998. PMID: 9692328 Review.
-
GALA: a designed synthetic pH-responsive amphipathic peptide with applications in drug and gene delivery.Adv Drug Deliv Rev. 2004 Apr 23;56(7):967-85. doi: 10.1016/j.addr.2003.10.041. Adv Drug Deliv Rev. 2004. PMID: 15066755 Review.
Cited by
-
Biologically active LIL proteins built with minimal chemical diversity.Proc Natl Acad Sci U S A. 2015 Aug 25;112(34):E4717-25. doi: 10.1073/pnas.1514230112. Epub 2015 Aug 10. Proc Natl Acad Sci U S A. 2015. PMID: 26261320 Free PMC article.
-
Structure and function of channels and channelogs as studied by computational chemistry.J Membr Biol. 1991 Jan;119(2):109-32. doi: 10.1007/BF01871411. J Membr Biol. 1991. PMID: 1710670 Review. No abstract available.
-
Charge delocalization in proton channels, I: the aquaporin channels and proton blockage.Biophys J. 2007 Jan 1;92(1):46-60. doi: 10.1529/biophysj.106.091934. Epub 2006 Oct 20. Biophys J. 2007. PMID: 17056733 Free PMC article.
-
Molecular dynamics of synthetic leucine-serine ion channels in a phospholipid membrane.Biophys J. 1999 Nov;77(5):2400-10. doi: 10.1016/S0006-3495(99)77077-3. Biophys J. 1999. PMID: 10545343 Free PMC article.
-
An amino-acid substitution in the influenza-B NB protein affects ion-channel gating.J Membr Biol. 2004 Feb 1;197(3):135-43. doi: 10.1007/s00232-004-0648-0. J Membr Biol. 2004. PMID: 15042345
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
