Punicalagin inhibits inflammation in LPS-induced RAW264.7 macrophages via the suppression of TLR4-mediated MAPKs and NF-κB activation
- PMID: 24473904
- DOI: 10.1007/s10753-014-9816-2
Punicalagin inhibits inflammation in LPS-induced RAW264.7 macrophages via the suppression of TLR4-mediated MAPKs and NF-κB activation
Abstract
Punicalagin (2,3,hexahydroxydiphenoyl-gallagyl-D-glucose and referred to as PUN) is a bioactive ellagitannin isolated from pomegranate, which is widely used for the treatment of inflammatory bowel disease (IBD), diarrhea, and ulcers in Chinese traditional medicine. In this study, we detected the anti-inflammation potentials of PUN in lipopolysaccharide (LPS)-induced macrophages and tried to uncover the underlying mechanism. Results demonstrated that PUN (25, 50, or 100 μM) treatment could significantly decrease the LPS-induced production of nitric oxide), prostaglandin E2 (PGE2), interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α in RAW264.7 cells. Molecular research showed that PUN inhibited the activation of upstream mediator nuclear factor-κB by suppressing the phosphorylation of IκBα and p65. Results also indicated that PUN could suppress the phosphorylation of mitogen-activated protein kinase including p38, c-Jun N-terminal kinase, and extracellular signal-regulated kinase. In conclusion, we observed that PUN could inhibit LPS-induced inflammation, and it may be a potential choice for the treatment of inflammation diseases.
Similar articles
-
Euscaphic acid isolated from roots of Rosa rugosa inhibits LPS-induced inflammatory responses via TLR4-mediated NF-κB inactivation in RAW 264.7 macrophages.J Cell Biochem. 2012 Jun;113(6):1936-46. doi: 10.1002/jcb.24062. J Cell Biochem. 2012. PMID: 22234926
-
Gallic Acid-g-Chitosan Modulates Inflammatory Responses in LPS-Stimulated RAW264.7 Cells Via NF-κB, AP-1, and MAPK Pathways.Inflammation. 2016 Feb;39(1):366-374. doi: 10.1007/s10753-015-0258-2. Inflammation. 2016. PMID: 26412258
-
Thymol inhibits LPS-stimulated inflammatory response via down-regulation of NF-κB and MAPK signaling pathways in mouse mammary epithelial cells.Inflammation. 2014 Feb;37(1):214-22. doi: 10.1007/s10753-013-9732-x. Inflammation. 2014. PMID: 24057926
-
Schisantherin A exhibits anti-inflammatory properties by down-regulating NF-kappaB and MAPK signaling pathways in lipopolysaccharide-treated RAW 264.7 cells.Inflammation. 2010 Apr;33(2):126-36. doi: 10.1007/s10753-009-9166-7. Inflammation. 2010. PMID: 20238486
-
2,8-Decadiene-1,10-Diol Inhibits Lipopolysaccharide-Induced Inflammatory Responses Through Inactivation of Mitogen-Activated Protein Kinase and Nuclear Factor-κB Signaling Pathway.Inflammation. 2016 Apr;39(2):583-91. doi: 10.1007/s10753-015-0283-1. Inflammation. 2016. PMID: 26610381
Cited by
-
Inhibitory Effect of Punicalagin on Inflammatory and Angiogenic Activation of Human Umbilical Vein Endothelial Cells.Front Pharmacol. 2021 Nov 16;12:727920. doi: 10.3389/fphar.2021.727920. eCollection 2021. Front Pharmacol. 2021. PMID: 34867335 Free PMC article.
-
Computational study of pomegranate peel extract polyphenols as potential inhibitors of SARS-CoV-2 virus internalization.Mol Cell Biochem. 2021 Feb;476(2):1179-1193. doi: 10.1007/s11010-020-03981-7. Epub 2020 Nov 16. Mol Cell Biochem. 2021. PMID: 33200379 Free PMC article.
-
Punicalagin as a novel selective aryl hydrocarbon receptor (AhR) modulator upregulates AhR expression through the PDK1/p90RSK/AP-1 pathway to promote the anti-inflammatory response and bactericidal activity of macrophages.Cell Commun Signal. 2024 Oct 3;22(1):473. doi: 10.1186/s12964-024-01847-9. Cell Commun Signal. 2024. PMID: 39363344 Free PMC article.
-
Lindera obtusiloba Attenuates Oxidative Stress and Airway Inflammation in a Murine Model of Ovalbumin-Challenged Asthma.Antioxidants (Basel). 2020 Jun 27;9(7):563. doi: 10.3390/antiox9070563. Antioxidants (Basel). 2020. PMID: 32605045 Free PMC article.
-
LPS Induces Preeclampsia-Like Phenotype in Rats and HTR8/SVneo Cells Dysfunction Through TLR4/p38 MAPK Pathway.Front Physiol. 2019 Aug 27;10:1030. doi: 10.3389/fphys.2019.01030. eCollection 2019. Front Physiol. 2019. PMID: 31507429 Free PMC article.
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials
Miscellaneous