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. 2013 Dec;15(12):1371-8.
doi: 10.1593/neo.131400.

Tumor evolution and intratumor heterogeneity of an oropharyngeal squamous cell carcinoma revealed by whole-genome sequencing

Xinyi Cindy Zhang  1 , Chang Xu  2 , Ryan M Mitchell  3 , Bo Zhang  1 , Derek Zhao  1 , Yao Li  1 , Xin Huang  1 , Wenhong Fan  1 , Hongwei Wang  1 , Luisa Angelica Lerma  3 , Melissa P Upton  4 , Ashley Hay  1 , Eduardo Méndez  5 , Lue Ping Zhao  6
Affiliations

Tumor evolution and intratumor heterogeneity of an oropharyngeal squamous cell carcinoma revealed by whole-genome sequencing

Xinyi Cindy Zhang et al. Neoplasia. 2013 Dec.

Abstract

Head and neck squamous cell carcinoma (HNSCC) is characterized by significant genomic instability that could lead to clonal diversity. Intratumor clonal heterogeneity has been proposed as a major attribute underlying tumor evolution, progression, and resistance to chemotherapy and radiation. Understanding genetic heterogeneity could lead to treatments specific to resistant and metastatic tumor cells. To characterize the degree of intratumor genetic heterogeneity within a single tumor, we performed whole-genome sequencing on three separate regions of an human papillomavirus (HPV)-positive oropharyngeal squamous cell carcinoma and two separate regions from one corresponding cervical lymph node metastasis. This approach achieved coverage of approximately 97.9% of the genome across all samples. In total, 5701 somatic point mutations (SPMs) and 4347 small somatic insertions and deletions (indels)were detected in at least one sample. Ninety-two percent of SPMs and 77% of indels were validated in a second set of samples adjacent to the discovery set. All five tumor samples shared 41% of SPMs, 57% of the 1805 genes with SPMs, and 34 of 55 cancer genes. The distribution of SPMs allowed phylogenetic reconstruction of this tumor's evolutionary pathway and showed that the metastatic samples arose as a late event. The degree of intratumor heterogeneity showed that a single biopsy may not represent the entire mutational landscape of HNSCC tumors. This approach may be used to further characterize intratumor heterogeneity in more patients, and their sample-to-sample variations could reveal the evolutionary process of cancer cells, facilitate our understanding of tumorigenesis, and enable the development of novel targeted therapies.

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Figures

Figure 1
Figure 1
Distribution of transition and transversion types of SPMs over the entire genome (A) and in the protein-coding region of the genome (B).
Figure 2
Figure 2
Venn diagram plot shows number of shared and unique genes with identified SPMs among batch “a” samples.
Figure 3
Figure 3
(A) Coalescent tree is based on number of shared genes containing SPMs. (B) Timeline of tumor progression based on estimated mutation rate indicates significant passage of time from the development of the initial cancerous cell to the development of the parental clone of all tumor samples obtained.
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