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. 2013 Dec 25:4:466.
doi: 10.3389/fimmu.2013.00466. eCollection 2013.

Huge Overlap of Individual TCR Beta Repertoires

Mikhail Shugay  1 , Dmitriy A Bolotin  1 , Ekaterina V Putintseva  1 , Mikhail V Pogorelyy  1 , Ilgar Z Mamedov  2 , Dmitriy M Chudakov  2
Affiliations

Huge Overlap of Individual TCR Beta Repertoires

Mikhail Shugay et al. Front Immunol. .
No abstract available

Keywords: NGS data analysis; TCR beta; TCR repertoire; adaptive immunity; overlap.

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Figures

Figure 1
Figure 1
Overlap of individual TCR beta CDR3 repertoires grows geometrically with the number of sequence pairs sampled. Plots indicate the number of shared sequences for 12 unrelated donor pairs in relation to sample size at the level of (A) all amino acid sequences, (B) amino acid sequence only, excluding matches with identical nucleotide sequences, and (C) nucleotide sequences. Each of the 12 colored lines represents the observed overlap between randomly drawn samples of unique CDR3 variants for a different pair of unrelated donors. To extrapolate the predicted level of overlap if the full individual TCR beta repertoires were to be sampled, we plotted fittings of averaged data with a power law (Y = aXb) as dashed lines. (D) We plotted the degree to which unique clonotypes were shared among our nine donors, and found that the frequency with which TCR beta clonotypes occur in human repertoires is distributed according to a power law.
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Comment on

  • A commentary on Mother and child T cell receptor repertoires: deep profiling study by Putintseva EV, Britanova OV, Staroverov DB, Merzlyak EM, Turchaninova MA, Shugay M, Bolotin DA, Pogorelyy MV, Mamedov IZ, Bobrynina V, Maschan M, Lebedev YB and Chudakov DM. Front Immunol (2013) 4:463. doi: 10.3389/fimmu.2013.00463

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References

    1. Robins HS, Srivastava SK, Campregher PV, Turtle CJ, Andriesen J, Riddell SR, et al. Overlap and effective size of the human CD8+ T cell receptor repertoire. Sci Transl Med (2010) 2:47ra64.10.1126/scitranslmed.3001442 - DOI - PMC - PubMed
    1. Venturi V, Quigley MF, Greenaway HY, Ng PC, Ende ZS, McIntosh T, et al. A mechanism for TCR sharing between T cell subsets and individuals revealed by pyrosequencing. J Immunol (2011) 186:4285–9410.4049/jimmunol.1003898 - DOI - PubMed
    1. Li H, Ye C, Ji G, Wu X, Xiang Z, Li Y, et al. Recombinatorial biases and convergent recombination determine interindividual TCRbeta sharing in murine thymocytes. J Immunol (2012) 189:2404–1310.4049/jimmunol.1102087 - DOI - PubMed
    1. Putintseva EV, Britanova OV, Staroverov DB, Merzlyak EM, Turchaninova MA, Shugay M, et al. Mother and child T cell receptor repertoires: deep profiling study. Front Immunol (2013) 4:463.10.3389/fimmu.2013.00463 - DOI - PMC - PubMed
    1. Robins HS, Campregher PV, Srivastava SK, Wacher A, Turtle CJ, Kahsai O, et al. Comprehensive assessment of T-cell receptor beta-chain diversity in alphabeta T cells. Blood (2009) 114:4099–10710.1182/blood-2009-04-217604 - DOI - PMC - PubMed

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